Sisymbrium officinale (L.) Scop. (hedge mustard) is a wild common plant of the Brassicaceae family. It is known as “the singers’ plant” for its traditional use in treating aphonia and vocal disability. The plant is rich in glucosinolates and isothiocyanates; the latter has been demonstrated to be a strong agonist in vitro of the Transient Receptor Potential Ankirine 1 (TRPA1) channel, which is involved in the somatosensory perception of pungency as well as in the nociception pathway of inflammatory pain. Volatile ITCs are released by the enzymatic or chemical hydrolysis of GLSs (glucosinolates) during sample crushing and/or by the mastication of fresh plant tissues when the plant is used as an ingredient. Some functional food and drink model preparations have been realised: honey enriched with seeds and flowers, infusions, cold drink (voice drink), artisanal beer, and a fermented tea (kombucha). Using SPME-GCMS chromatography, we analysed samples of the plant and of the food preparations adopting conditions that simulate the release of isothiocyanates (ITCs) during oral assumption. Two active compounds, iso-propylisothiocyanate and 2-butylisothiocyanate, have been assayed. The concentration of ITCs varies according to temperature, pH, grinding conditions, and different plant organs used. Kombucha-type fermentation seems to eliminate the ITCs, whereas they are retained in beer. The ITCs’ concentration is higher when entire seeds and flowers are used.

Sisymbrium officinale (the singers’ plant) as an ingredient : analysis of somatosensory active volatile isothiocyanates in model food and drinks / P. De Nisi, G. Borgonovo, S. Tramontana, S. Grassi, C. Picozzi, L. Scaglioni, S. Mazzini, N. Mangieri, A. Bassoli. - In: FOODS. - ISSN 2304-8158. - 10:2(2021 Feb 03), pp. 308.1-308.12. [10.3390/foods10020308]

Sisymbrium officinale (the singers’ plant) as an ingredient : analysis of somatosensory active volatile isothiocyanates in model food and drinks

P. De Nisi
Primo
Methodology
;
G. Borgonovo
Secondo
Methodology
;
S. Grassi
Writing – Review & Editing
;
C. Picozzi
Methodology
;
L. Scaglioni
Formal Analysis
;
S. Mazzini
Formal Analysis
;
N. Mangieri
Penultimo
Formal Analysis
;
A. Bassoli
Ultimo
Conceptualization
2021

Abstract

Sisymbrium officinale (L.) Scop. (hedge mustard) is a wild common plant of the Brassicaceae family. It is known as “the singers’ plant” for its traditional use in treating aphonia and vocal disability. The plant is rich in glucosinolates and isothiocyanates; the latter has been demonstrated to be a strong agonist in vitro of the Transient Receptor Potential Ankirine 1 (TRPA1) channel, which is involved in the somatosensory perception of pungency as well as in the nociception pathway of inflammatory pain. Volatile ITCs are released by the enzymatic or chemical hydrolysis of GLSs (glucosinolates) during sample crushing and/or by the mastication of fresh plant tissues when the plant is used as an ingredient. Some functional food and drink model preparations have been realised: honey enriched with seeds and flowers, infusions, cold drink (voice drink), artisanal beer, and a fermented tea (kombucha). Using SPME-GCMS chromatography, we analysed samples of the plant and of the food preparations adopting conditions that simulate the release of isothiocyanates (ITCs) during oral assumption. Two active compounds, iso-propylisothiocyanate and 2-butylisothiocyanate, have been assayed. The concentration of ITCs varies according to temperature, pH, grinding conditions, and different plant organs used. Kombucha-type fermentation seems to eliminate the ITCs, whereas they are retained in beer. The ITCs’ concentration is higher when entire seeds and flowers are used.
sisymbrium officinale; isothiocyanates; HS-SPME-GC/MS; TRPA1 ion channel; somatosensory; trigeminal perception
Settore CHIM/06 - Chimica Organica
Settore CHIM/10 - Chimica degli Alimenti
Settore BIO/10 - Biochimica
Settore AGR/15 - Scienze e Tecnologie Alimentari
Erisimo a Milano
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/812699
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