Short chain fatty acids (SCFAs), especially butyrate (BUT), are known to promote intestinal health, but their role in the protection of intestinal barrier integrity is poorly characterized. The aim of the study was to set up an in vitro model of human colon epithelium using HT29-MTX-E12 cells to delineate the potential role of SCFAs under stress conditions. Accordingly, the HT29-MTX-E12 cells were differentiated for 42 days and subsequently exposed to dextran sulphate sodium (DSS). Further, the effects of BUT or its mixture with acetate and propionate (SCFAs-MIX) were tested to study proliferation, epithelial integrity and mucus production. The results showed that the concentration of 10% DSS for 24 h decreased the TEER about 50% compared to the control in HT29-MTX-E12 cells. The pre-treatment on HT29-MTX-E12 cells with BUT or SCFAs-MIX at specific concentrations significantly (p < 0.05) reduced the DSS-induced damage on epithelial cell integrity and permeability. Further, the treatment with specific concentrations of BUT and SCFAs-MIX for 24 h significantly promoted ZO-1, MUC2 and MUC5AC mRNA expression (p < 0.005). The present study demonstrated the suitability of HT29-MTX-E12 cells treated with DSS as an in vitro stress model of inflammatory bowel disease, which enabled us to understand the effect of bioactive SCFAs on the intestinal barrier.

Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells / C. Giromini, A. Baldi, R. Rebucci, D. Lanzoni, M. Policardi, T.S. Sundaram, S. Purup. - In: FOODS. - ISSN 2304-8158. - 11:13(2022 Jul 05), pp. 1983.1-1983.13. [10.3390/foods11131983]

Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells

C. Giromini
Primo
;
A. Baldi
Secondo
;
R. Rebucci;D. Lanzoni;T.S. Sundaram
Penultimo
;
2022

Abstract

Short chain fatty acids (SCFAs), especially butyrate (BUT), are known to promote intestinal health, but their role in the protection of intestinal barrier integrity is poorly characterized. The aim of the study was to set up an in vitro model of human colon epithelium using HT29-MTX-E12 cells to delineate the potential role of SCFAs under stress conditions. Accordingly, the HT29-MTX-E12 cells were differentiated for 42 days and subsequently exposed to dextran sulphate sodium (DSS). Further, the effects of BUT or its mixture with acetate and propionate (SCFAs-MIX) were tested to study proliferation, epithelial integrity and mucus production. The results showed that the concentration of 10% DSS for 24 h decreased the TEER about 50% compared to the control in HT29-MTX-E12 cells. The pre-treatment on HT29-MTX-E12 cells with BUT or SCFAs-MIX at specific concentrations significantly (p < 0.05) reduced the DSS-induced damage on epithelial cell integrity and permeability. Further, the treatment with specific concentrations of BUT and SCFAs-MIX for 24 h significantly promoted ZO-1, MUC2 and MUC5AC mRNA expression (p < 0.005). The present study demonstrated the suitability of HT29-MTX-E12 cells treated with DSS as an in vitro stress model of inflammatory bowel disease, which enabled us to understand the effect of bioactive SCFAs on the intestinal barrier.
English
dietary fibre; fatty acids; gut health; mucus layer
Settore AGR/15 - Scienze e Tecnologie Alimentari
Articolo
Esperti anonimi
Pubblicazione scientifica
   Joint Doctorate in Molecular Animal Nutrition (MANNA)
   MANNA
   EUROPEAN COMMISSION
   H2020
   765423
5-lug-2022
MDPI
11
13
1983
1
13
13
Pubblicato
Periodico con rilevanza internazionale
pubmed
wos
scopus
crossref
Aderisco
info:eu-repo/semantics/article
Role of Short Chain Fatty Acids to Counteract Inflammatory Stress and Mucus Production in Human Intestinal HT29-MTX-E12 Cells / C. Giromini, A. Baldi, R. Rebucci, D. Lanzoni, M. Policardi, T.S. Sundaram, S. Purup. - In: FOODS. - ISSN 2304-8158. - 11:13(2022 Jul 05), pp. 1983.1-1983.13. [10.3390/foods11131983]
open
Prodotti della ricerca::01 - Articolo su periodico
7
262
Article (author)
si
C. Giromini, A. Baldi, R. Rebucci, D. Lanzoni, M. Policardi, T.S. Sundaram, S. Purup
File in questo prodotto:
File Dimensione Formato  
foods-11-01983.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 3.99 MB
Formato Adobe PDF
3.99 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/944448
Citazioni
  • ???jsp.display-item.citation.pmc??? 3
  • Scopus 8
  • ???jsp.display-item.citation.isi??? 7
social impact