Adzuki seed -vignin, a vicilin-like globulin, has proven to exert various health-promoting biological activities, notably in cardiovascular health. A simple scalable enrichment procedure of this protein for further nutritional and functional studies is crucial. In this study, a simplified chromatography-independent protein fractionation procedure has been optimized and described. The electrophoretic analysis showed a high degree of homogeneity of -vignin isolate. Furthermore, the molecular features of the purified protein were investigated. The adzuki bean -vignin was found to have a native size of 146 kDa, and the molecular weight determined was consistent with a trimeric structure. These were identified in two main polypeptide chains (masses of 56–54 kDa) that are glycosylated polypeptides with metal binding capacity, and one minor polypeptide chain with a mass 37 kDa, wherein these features are absent. The in vitro analysis showed a high degree of digestibility of the protein (92%) and potential anti-inflammatory capacity. The results lay the basis not only for further investigation of the health-promoting properties of the adzuki bean -vignin protein, but also for a possible application as nutraceutical molecule.
Chromatography-independent fractionation and newly identified molecular features of the Adzuki Bean (Vigna angularis Willd.) β-vignin protein / B. Philadelpho, V. Souza, F. Souza, J. Santos, F. Batista, M. Silva, J. Capraro, S. De Benedetti, G.C. Heinzl, E. Cilli, A. Scarafoni, C. Magni, E. Ferreira. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - 22:6(2021 Mar 16), pp. 3018.1-3018.14.
|Titolo:||Chromatography-independent fractionation and newly identified molecular features of the Adzuki Bean (Vigna angularis Willd.) β-vignin protein|
MAGNI, CHIARA (Corresponding)
|Parole Chiave:||protein vicilin-type; protein fractionation; biological activities; in vitro digestibility; amino acid sequencing; glycosylated polypeptides; metal binding capacity|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||16-mar-2021|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/ijms22063018|
|Appare nelle tipologie:||01 - Articolo su periodico|