Cowpea seed β-vignin, a vicilin-like globulin, proved to exert various health favourable effects, including blood cholesterol reduction in animal models. The need of a simple scalable enrichment procedure for further studies for tailored applications of this seed protein is crucial. A chromatography-independent fractionation method allowing to obtain a protein preparation with a high degree of homogeneity was used. Further purification was pursued to deep the molecular characterisation of β-vignin. The results showed: (i) differing glycosylation patterns of the two constituent polypeptides, in agreement with amino acid sequence features; (ii) the seed accumulation of a gene product never identified before; (iii) metal binding capacity of native protein, a property observed only in few other legume seed vicilins.
New molecular features of cowpea bean (Vigna unguiculata, l. Walp) β-vignin / F. Ederlan de Souza, J. Capraro, F. Sessa, C. Magni, D. Aureluce, A. Consonni, N. Valdir Augusto, M. Cilli Eduardo, M. Duranti, A. Scarafoni. - In: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY. - ISSN 0916-8451. - 82:2(2018 Feb), pp. 285-291. [10.1080/09168451.2017.1419855]
New molecular features of cowpea bean (Vigna unguiculata, l. Walp) β-vignin
J. Capraro
;F. Sessa;C. Magni;A. Consonni;M. Duranti;A. ScarafoniUltimo
2018
Abstract
Cowpea seed β-vignin, a vicilin-like globulin, proved to exert various health favourable effects, including blood cholesterol reduction in animal models. The need of a simple scalable enrichment procedure for further studies for tailored applications of this seed protein is crucial. A chromatography-independent fractionation method allowing to obtain a protein preparation with a high degree of homogeneity was used. Further purification was pursued to deep the molecular characterisation of β-vignin. The results showed: (i) differing glycosylation patterns of the two constituent polypeptides, in agreement with amino acid sequence features; (ii) the seed accumulation of a gene product never identified before; (iii) metal binding capacity of native protein, a property observed only in few other legume seed vicilins.File | Dimensione | Formato | |
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