Intestinal inflammation is a natural process crucial for the maintenance of gut functioning. However, abnormal or prolonged inflammatory responses may lead to the onset of chronic degenerative diseases, typically treated by means of pharmacological interventions. Dietary strategies for the prevention of inflammation are a safer alternative to pharmacotherapy. Anthocyanins and other polyphenols have been documented to display anti-inflammatory activity. In the present study, three bioactive fractions (anthocyanin, phenolic, and water-soluble fractions) were extracted from a wild blueberry powder. The Caco-2 intestinal model was used to test the immunomodulatory effect of the above fractions. Only the anthocyanin-rich fraction reduced the activation of NF-κB, induced by IL-1β in intestinal epithelial Caco-2 cells. Specifically, concentrations of 50 and 100 μg mL(-1) decreased NF-κB activation by 68.9 and 85.2%, respectively (p ≤ 0.05). These preliminary results provide further support for the role of food bioactives as potential dietary anti-inflammatory agents.

Immunomodulatory effect of a wild blueberry anthocyanin-rich extract in human Caco-2 intestinal cells / V. Taverniti, D. Fracassetti, C. Del Bo', C. Lanti, M. Minuzzo, D. Klimis Zacas, P. Riso, S. Guglielmetti. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - 62:33(2014 Aug 20), pp. 8346-8351. [10.1021/jf502180j]

Immunomodulatory effect of a wild blueberry anthocyanin-rich extract in human Caco-2 intestinal cells

V. Taverniti
Primo
;
D. Fracassetti
Secondo
;
C. Del Bo';C. Lanti;M. Minuzzo;P. Riso
;
S. Guglielmetti
Ultimo
2014

Abstract

Intestinal inflammation is a natural process crucial for the maintenance of gut functioning. However, abnormal or prolonged inflammatory responses may lead to the onset of chronic degenerative diseases, typically treated by means of pharmacological interventions. Dietary strategies for the prevention of inflammation are a safer alternative to pharmacotherapy. Anthocyanins and other polyphenols have been documented to display anti-inflammatory activity. In the present study, three bioactive fractions (anthocyanin, phenolic, and water-soluble fractions) were extracted from a wild blueberry powder. The Caco-2 intestinal model was used to test the immunomodulatory effect of the above fractions. Only the anthocyanin-rich fraction reduced the activation of NF-κB, induced by IL-1β in intestinal epithelial Caco-2 cells. Specifically, concentrations of 50 and 100 μg mL(-1) decreased NF-κB activation by 68.9 and 85.2%, respectively (p ≤ 0.05). These preliminary results provide further support for the role of food bioactives as potential dietary anti-inflammatory agents.
wild blueberry; anthocyanins; Caco-2 cells; NP-kappa B; luciferase reporter system
Settore MED/49 - Scienze Tecniche Dietetiche Applicate
Article (author)
File in questo prodotto:
File Dimensione Formato  
JAFC 2014.pdf

accesso riservato

Descrizione: Articolo
Tipologia: Publisher's version/PDF
Dimensione 283.59 kB
Formato Adobe PDF
283.59 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Taverniti et al., post print.pdf

accesso aperto

Descrizione: Articolo Principale
Tipologia: Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione 1.05 MB
Formato Adobe PDF
1.05 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/246105
Citazioni
  • ???jsp.display-item.citation.pmc??? 16
  • Scopus 66
  • ???jsp.display-item.citation.isi??? 60
social impact