Objective: To analyze the effect of the juice obtained from two varieties of sweet orange (Citrus sinensis L. Osbeck), Moro (a blood orange) and Navelina (a blond orange), on fat accumulation in mice fed a standard or a high-fat diet (HFD). Methods: Obesity was induced in male C57/Bl6 mice by feeding a HFD. Moro and Navelina juices were provided instead of water. The effect of an anthocyanin-enriched extract from Moro oranges or purified cyanidin-3-glucoside (C3G) was also analyzed. Body weight and food intake were measured regularly over a 12-week period. The adipose pads were weighted and analyzed histologically; total RNA was also isolated for microarray analysis. Results: Dietary supplementation of Moro juice, but not Navelina juice significantly reduced body weight gain and fat accumulation regardless of the increased energy intake because of sugar content. Furthermore, mice drinking Moro juice were resistant to HFD-induced obesity with no alterations in food intake. Only the anthocyanin extract, but not the purified C3G, slightly affected fat accumulation. High-throughput gene expression analysis of fat tissues confirmed that Moro juice could entirely rescue the high fat-induced transcriptional reprogramming. Conclusion: Moro juice anti-obesity effect on fat accumulation cannot be explained only by its anthocyanin content. Our findings suggest that multiple components present in the Moro orange juice might act synergistically to inhibit fat accumulation.

Blood orange juice inhibits fat accumulation in mice / L. Titta, M. Trinei, M. Stendardo, I. Berniakovich, K. Petroni, C. Tonelli, P. Riso, M. Porrini, S. Minucci, P.G. Pelicci, P. Rapisarda, G. Reforgiato Recupero, M. Giorgio. - In: INTERNATIONAL JOURNAL OF OBESITY. - ISSN 0307-0565. - 34:3(2010), pp. 578-588. [10.1038/ijo.2009.266]

Blood orange juice inhibits fat accumulation in mice

L. Titta
Primo
;
K. Petroni;C. Tonelli;P. Riso;M. Porrini;S. Minucci;P.G. Pelicci;
2010

Abstract

Objective: To analyze the effect of the juice obtained from two varieties of sweet orange (Citrus sinensis L. Osbeck), Moro (a blood orange) and Navelina (a blond orange), on fat accumulation in mice fed a standard or a high-fat diet (HFD). Methods: Obesity was induced in male C57/Bl6 mice by feeding a HFD. Moro and Navelina juices were provided instead of water. The effect of an anthocyanin-enriched extract from Moro oranges or purified cyanidin-3-glucoside (C3G) was also analyzed. Body weight and food intake were measured regularly over a 12-week period. The adipose pads were weighted and analyzed histologically; total RNA was also isolated for microarray analysis. Results: Dietary supplementation of Moro juice, but not Navelina juice significantly reduced body weight gain and fat accumulation regardless of the increased energy intake because of sugar content. Furthermore, mice drinking Moro juice were resistant to HFD-induced obesity with no alterations in food intake. Only the anthocyanin extract, but not the purified C3G, slightly affected fat accumulation. High-throughput gene expression analysis of fat tissues confirmed that Moro juice could entirely rescue the high fat-induced transcriptional reprogramming. Conclusion: Moro juice anti-obesity effect on fat accumulation cannot be explained only by its anthocyanin content. Our findings suggest that multiple components present in the Moro orange juice might act synergistically to inhibit fat accumulation.
Anthocyanins; Anti-obesity; Blood orange; Cyanidin-3-glucoside; Fat accumulation; Orange juice
Settore BIO/18 - Genetica
2010
Article (author)
File in questo prodotto:
File Dimensione Formato  
Titta et al., 2010-Moro orange.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 688.14 kB
Formato Adobe PDF
688.14 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/144389
Citazioni
  • ???jsp.display-item.citation.pmc??? 50
  • Scopus 130
  • ???jsp.display-item.citation.isi??? 112
social impact