Streptomycetes show promising attributes as biocontrol agents: they are soil dwellers and plant symbionts able to produce a plethora of specialised metabolites with antibiotic and antifungal activities. Efficiency of the spore production process is a fundamental step leading to large scale application of promising biocontrol strains. In this study, Streptomyces spp. DEF-A and DEF-B, previously characterized as biocontrol agents (BCA) against Fusarium Head Blight on wheat, were tested for high spore and biomass production in solid state fermentation (SSF). SSF is a biotechnological process in which microorganisms grow in absence or near-absence of flow-free water. Recently, this process had re-gained attention due to the possibility to use raw materials and by-products as SSF substrate to produce value-added products such as enzymes and organic acids. Few studies have focused on mass production of spores or BCAs production using waste-based substrates. To evaluate the DEF-A and DEF-B biomass production in SSF, the two strains were grown on defatted wheat bran alone or mixed with soil. Interestingly, the two strains had different ability to colonize and grow on the substrates reaching the highest concentration respectively on wheat bran (DEF-A, 8 × 108 CFU/g) and on wheat bran mixed with soil (DEF-B, 2.9 × 109 CFU/g). The strains grown in SSF were formulated and tested in vivo for their biocontrol potential against Fusarium Foot and Root Rot caused by Fusarium culmorum. The application of each Streptomyces strain (106 CFU) in the soil during wheat sowing improved seedling emergence by 18.9 and 22.4% at maximum Fusarium disease pressure (no survival of the control parcels). This percentage increase to 49% in the presence of higher concentration of DEF-A (108 CFU). The results propose SSF as an innovative method for mass-production of biocontrol agents on waste-based substrates and potentially expands the application of the two BCAs to other pathosystems.

Streptomyces spp. in solid state fermentation: biocontrol activity against Fusarium culmorum / D. Bulgari, L. Pisoni, M. Saracchi, C. Pizzatti, A. Kunova, P. Cortesi, M. Pasquali. ((Intervento presentato al convegno International symposium miCROPe2024 : Microbe-assisted crop production - opportunities, challenges and needs: 15-18 july tenutosi a Vienna nel 2024.

Streptomyces spp. in solid state fermentation: biocontrol activity against Fusarium culmorum

D. Bulgari;L. Pisoni;M. Saracchi;C. Pizzatti;A. Kunova;P. Cortesi;M. Pasquali
2024

Abstract

Streptomycetes show promising attributes as biocontrol agents: they are soil dwellers and plant symbionts able to produce a plethora of specialised metabolites with antibiotic and antifungal activities. Efficiency of the spore production process is a fundamental step leading to large scale application of promising biocontrol strains. In this study, Streptomyces spp. DEF-A and DEF-B, previously characterized as biocontrol agents (BCA) against Fusarium Head Blight on wheat, were tested for high spore and biomass production in solid state fermentation (SSF). SSF is a biotechnological process in which microorganisms grow in absence or near-absence of flow-free water. Recently, this process had re-gained attention due to the possibility to use raw materials and by-products as SSF substrate to produce value-added products such as enzymes and organic acids. Few studies have focused on mass production of spores or BCAs production using waste-based substrates. To evaluate the DEF-A and DEF-B biomass production in SSF, the two strains were grown on defatted wheat bran alone or mixed with soil. Interestingly, the two strains had different ability to colonize and grow on the substrates reaching the highest concentration respectively on wheat bran (DEF-A, 8 × 108 CFU/g) and on wheat bran mixed with soil (DEF-B, 2.9 × 109 CFU/g). The strains grown in SSF were formulated and tested in vivo for their biocontrol potential against Fusarium Foot and Root Rot caused by Fusarium culmorum. The application of each Streptomyces strain (106 CFU) in the soil during wheat sowing improved seedling emergence by 18.9 and 22.4% at maximum Fusarium disease pressure (no survival of the control parcels). This percentage increase to 49% in the presence of higher concentration of DEF-A (108 CFU). The results propose SSF as an innovative method for mass-production of biocontrol agents on waste-based substrates and potentially expands the application of the two BCAs to other pathosystems.
lug-2024
Settore AGRI-05/B - Patologia vegetale
https://www.micrope.org/
Streptomyces spp. in solid state fermentation: biocontrol activity against Fusarium culmorum / D. Bulgari, L. Pisoni, M. Saracchi, C. Pizzatti, A. Kunova, P. Cortesi, M. Pasquali. ((Intervento presentato al convegno International symposium miCROPe2024 : Microbe-assisted crop production - opportunities, challenges and needs: 15-18 july tenutosi a Vienna nel 2024.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1124268
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