Buckwheat is a summer growing pseudocereal that has attracted considerable interest thanks to its excellent nutritional value and low environmental impact. Buckwheat requires low doses of fertilizers and does not need pesticides. Noteworthily, buckwheat is a healthy alternative to gluten-containing grain. The first step of buckwheat processing is husk removal through decortication, a process that produces a significant amount of byproducts. Buckwheat husk has shown to be a rich source of bioactives as well as of various other materials, including cellulose and lignocellulose fractions. The recovery of these species can be beneficial to human nutrition or exploited in the context of many industrial applications. The objective of this study is to propose a “green” valorisation method for the bioactive recovery from buckwheat husk, by exploiting different extraction methods, such as Ultrasound Assisted Extraction (UAE) and Microwave Assisted Extraction (MAE). These procedures have been evaluated by using water-based media as the solvent, and results have been compared to one of the traditional extraction methods, involving the use of acidified methanol. The bioactive molecules extracted in both conditions were characterized in terms of profile, relative abundance, and antioxidant activity, showing improved overall yields and a higher content of relevant bioactive species in the “green” extracts. The potential protective effects of bioactive compounds on cell inflammation has been studied on human Caco-2 cells, indicating significant effects of the “green” extracts on selected inflammation biomarkers. Information from these studies will allow to define the possible use(s) of individual materials in food and non-food products.
A green path to the recovery of bioactive molecules from buckwheat husk / A.R. Speranza, G. Heinzl, A. Scarafoni, A.G. Barbiroli, S. Iametti. ((Intervento presentato al 62. convegno Congresso SIB (Società Italiana Biochimica) tenutosi a Firenze nel 2023.
A green path to the recovery of bioactive molecules from buckwheat husk
A.R. Speranza
;G. Heinzl
;A. Scarafoni
;A.G. Barbiroli
;S. Iametti
2023
Abstract
Buckwheat is a summer growing pseudocereal that has attracted considerable interest thanks to its excellent nutritional value and low environmental impact. Buckwheat requires low doses of fertilizers and does not need pesticides. Noteworthily, buckwheat is a healthy alternative to gluten-containing grain. The first step of buckwheat processing is husk removal through decortication, a process that produces a significant amount of byproducts. Buckwheat husk has shown to be a rich source of bioactives as well as of various other materials, including cellulose and lignocellulose fractions. The recovery of these species can be beneficial to human nutrition or exploited in the context of many industrial applications. The objective of this study is to propose a “green” valorisation method for the bioactive recovery from buckwheat husk, by exploiting different extraction methods, such as Ultrasound Assisted Extraction (UAE) and Microwave Assisted Extraction (MAE). These procedures have been evaluated by using water-based media as the solvent, and results have been compared to one of the traditional extraction methods, involving the use of acidified methanol. The bioactive molecules extracted in both conditions were characterized in terms of profile, relative abundance, and antioxidant activity, showing improved overall yields and a higher content of relevant bioactive species in the “green” extracts. The potential protective effects of bioactive compounds on cell inflammation has been studied on human Caco-2 cells, indicating significant effects of the “green” extracts on selected inflammation biomarkers. Information from these studies will allow to define the possible use(s) of individual materials in food and non-food products.File | Dimensione | Formato | |
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