Beta-casomorphin-7 (BCM7) represents the fragment Val60-Ile66 of bovine β-casein (β-CN), and there is evidence that it is more easily released during gastrointestinal digestion (GID) of A1 β-CN variant, in comparison to the A2 variant. This study aimed at investigating the effect of type of enzymes and the protease/protein (P/S) ratio on BCM7 release during the intestinal step of in vitro static GID of bovine milk and cheeses with A1 or A2 β-CN phenotypes. BCM7 occurred in digests of both A1 and A2 samples, being the release more marked for A1 counterparts. Nonetheless, the BCM7 release depended on both the type of GID enzymes and the P/S ratio. These findings highlight the importance of GID conditions which may affect the outcomes for possible differences between A1 and A2 milk based on BCM7 release during in vitro GID.

Impact of in vitro static digestion method on the release of β-casomorphin-7 from bovine milk and cheeses with A1 or A2 β-casein phenotypes / S. Cattaneo, F. Masotti, M. Stuknyte, I. DE NONI. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 404:pt. A(2023 Mar 15), pp. 134617.1-134617.7. [10.1016/j.foodchem.2022.134617]

Impact of in vitro static digestion method on the release of β-casomorphin-7 from bovine milk and cheeses with A1 or A2 β-casein phenotypes

S. Cattaneo
Primo
;
F. Masotti
Secondo
;
M. Stuknyte
Penultimo
;
I. DE NONI
Ultimo
2023

Abstract

Beta-casomorphin-7 (BCM7) represents the fragment Val60-Ile66 of bovine β-casein (β-CN), and there is evidence that it is more easily released during gastrointestinal digestion (GID) of A1 β-CN variant, in comparison to the A2 variant. This study aimed at investigating the effect of type of enzymes and the protease/protein (P/S) ratio on BCM7 release during the intestinal step of in vitro static GID of bovine milk and cheeses with A1 or A2 β-CN phenotypes. BCM7 occurred in digests of both A1 and A2 samples, being the release more marked for A1 counterparts. Nonetheless, the BCM7 release depended on both the type of GID enzymes and the P/S ratio. These findings highlight the importance of GID conditions which may affect the outcomes for possible differences between A1 and A2 milk based on BCM7 release during in vitro GID.
A1 and A2 bovine β-casein; Cheese; In vitro static gastrointestinal digestion; Milk; β-Casomorphin-7;
Settore AGR/15 - Scienze e Tecnologie Alimentari
15-mar-2023
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/968998
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