Hamamelis virginiana L. bark extract is a traditional remedy for skin affections, including atopic dermatitis/eczema (AD). Hamamelis preparations contain tannins, including hamamelitannin (HT), although their pharmacological role in AD is still unknown. This study aimed to study the rational for its topical use by considering the impact of crucial biomarkers on AD pathogenesis. A standardized extract (HVE) (0.5-125 mu g/mL) was compared to hamamelitannin (HT), its main compound (0.5-5 mu g/mL), in a model of human keratinocytes (HaCaTs), challenged with an AD-like cytokine milieu (TNF-alpha, IFN-gamma, and IL-4). HVE inhibited the release of mediators involved in skin autoimmunity (IL-6 and IL-17C) and allergy (TSLP, IL-6, CCL26, and MMP-9) with a concentration-dependent fashion (IC50s < 25 mu g/mL). The biological mechanism was ascribed, at least in part, to the impairment of the NF-kappa B-driven transcription. Moreover, HVE counteracted the proliferative effects of IL-4 and recovered K10, a marker of skin differentiation. Notably, HT showed activity on well-known targets of IL-4 pathway (CCL26, K10, cell proliferation). To the best of our knowledge, this work represents the first demonstration of the potential role of Hamamelis virginiana in the control of AD symptoms, such as itch and skin barrier impairment, supporting the relevance of the whole phytocomplex.

Unveiling the Ability of Witch Hazel (Hamamelis virginiana L.) Bark Extract to Impair Keratinocyte Inflammatory Cascade Typical of Atopic Eczema / S. Piazza, G. Martinelli, A. Magnavacca, M. Fumagalli, C. Pozzoli, M. Terno, L. Canilli, M. Angarano, N. Maranta, M. Dell'Agli, E. Sangiovanni. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 23:16(2022 Aug 17), pp. 9279.1-9279.15. [10.3390/ijms23169279]

Unveiling the Ability of Witch Hazel (Hamamelis virginiana L.) Bark Extract to Impair Keratinocyte Inflammatory Cascade Typical of Atopic Eczema

S. Piazza
Primo
;
G. Martinelli
Secondo
;
A. Magnavacca;M. Fumagalli;C. Pozzoli;M. Dell'Agli
Penultimo
;
E. Sangiovanni
Ultimo
2022

Abstract

Hamamelis virginiana L. bark extract is a traditional remedy for skin affections, including atopic dermatitis/eczema (AD). Hamamelis preparations contain tannins, including hamamelitannin (HT), although their pharmacological role in AD is still unknown. This study aimed to study the rational for its topical use by considering the impact of crucial biomarkers on AD pathogenesis. A standardized extract (HVE) (0.5-125 mu g/mL) was compared to hamamelitannin (HT), its main compound (0.5-5 mu g/mL), in a model of human keratinocytes (HaCaTs), challenged with an AD-like cytokine milieu (TNF-alpha, IFN-gamma, and IL-4). HVE inhibited the release of mediators involved in skin autoimmunity (IL-6 and IL-17C) and allergy (TSLP, IL-6, CCL26, and MMP-9) with a concentration-dependent fashion (IC50s < 25 mu g/mL). The biological mechanism was ascribed, at least in part, to the impairment of the NF-kappa B-driven transcription. Moreover, HVE counteracted the proliferative effects of IL-4 and recovered K10, a marker of skin differentiation. Notably, HT showed activity on well-known targets of IL-4 pathway (CCL26, K10, cell proliferation). To the best of our knowledge, this work represents the first demonstration of the potential role of Hamamelis virginiana in the control of AD symptoms, such as itch and skin barrier impairment, supporting the relevance of the whole phytocomplex.
Hamamelis virginiana; allergy; atopic dermatitis; eczema; phytotherapy; witch hazel; Cytokines; Humans; Interleukin-4; Interleukin-6; Keratinocytes; Plant Bark; Plant Extracts; Skin; Dermatitis, Atopic; Hamamelis
Settore BIO/14 - Farmacologia
DECC18ACORS_01 - Dipartimenti di Eccellenza 2018-2022 - Dipartimento di SCIENZE FARMACOLOGICHE E BIOMOLECOLARI - CORSINI, ALBERTO - DECC - Bando Dipartimenti di Eccellenza - 2018
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/938208
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