Optimized recombinant whole cells of E. coli bearing CYP153A6 were employed for catalyzing the hydroxylation of different monoterpene derivatives. In most cases, high selectivity was observed with exclusive hydroxylation of the allylic methyl group bound to the aliphatic ring. In the case of (R)- and (S)-carvone, hydroxylation occurred also on the other allylic methyl group, although to a lesser extent. Biotransformations carried out in fed-batch mode on (S)-limonene and α-terpineol showed that recombinant whole cells retained activity for at least 24 h, allowing for the recovery of 3.25 mg mL−1 of (S)-perillyl alcohol and 5.45 mg mL−1 of 7-hydroxy-α-terpineol, respectively.
Whole cells of recombinant CYP153A6-E. coli as biocatalyst for regioselective hydroxylation of monoterpenes / P. Cannazza, M. Rabuffetti, S. Donzella, V. De Vitis, M.L. Contente, M.C.F. de Oliveira, M.C. de Mattos, F.G. Barbosa, R.P. de Souza Oliveira, A. Pinto, F. Molinari, D. Romano. - In: AMB EXPRESS. - ISSN 2191-0855. - 12:1(2022 Apr 27), pp. 48.1-48.9. [10.1186/s13568-022-01389-8]
Whole cells of recombinant CYP153A6-E. coli as biocatalyst for regioselective hydroxylation of monoterpenes
P. Cannazza;M. Rabuffetti;S. Donzella;V. De Vitis;M.L. Contente;A. Pinto;F. Molinari;D. Romano
2022
Abstract
Optimized recombinant whole cells of E. coli bearing CYP153A6 were employed for catalyzing the hydroxylation of different monoterpene derivatives. In most cases, high selectivity was observed with exclusive hydroxylation of the allylic methyl group bound to the aliphatic ring. In the case of (R)- and (S)-carvone, hydroxylation occurred also on the other allylic methyl group, although to a lesser extent. Biotransformations carried out in fed-batch mode on (S)-limonene and α-terpineol showed that recombinant whole cells retained activity for at least 24 h, allowing for the recovery of 3.25 mg mL−1 of (S)-perillyl alcohol and 5.45 mg mL−1 of 7-hydroxy-α-terpineol, respectively.File | Dimensione | Formato | |
---|---|---|---|
Cannazza2022_Article_WholeCellsOfRecombinantCYP153A.pdf
accesso aperto
Tipologia:
Publisher's version/PDF
Dimensione
1.28 MB
Formato
Adobe PDF
|
1.28 MB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.