Quebracho (Qu) and chestnut (Ch) are natural sources of tannins and they are currently used in animal nutrition as feed ingredients. However, to date the bio-accessibility, antimicrobial, antioxidant, and intestinal epithelial cell stimulatory doses of Qu and Ch have not been determined. Our study investigates the antioxidant and E. coli F4+ and F18+ growth inhibitory activity of Qu, Ch, and their combinations after solubilization in water (to evaluate the already bio-accessible molecules) and after simulated gastro-intestinal digestion in vitro. The effect of an in vitro digested Ch and Qu combination was also tested on intestinal epithelial IPEC-J2 cells experimentally stressed with hydrogen peroxide (H2O2) and Dextran Sodium Sulfate (DSS). The results showed that undigested Qu and Ch alone, and in combination, exerted a valuable antioxidant capacity and E. coli F4+ and F18+ growth inhibitory activity. The concentration of 1200 μg/mL exhibited the highest E. coli growth inhibitory activity for all the samples tested. In addition, after in vitro digestion, Qu and Qu50%–Ch50% maintained E. coli growth inhibitory activity and a modest antioxidant capacity. Three hours pre-treatment with in vitro digested Qu50%–Ch50% counteracted the H2O2 and DSS experimentally-induced stress in the intestinal IPEC-J2 cells. Ch and Qu tannin extracts, particularly when combined, may exert E. coli F4+ and F18+ growth inhibitory activity and valuable antioxidant and cell viability modulation activities.
In Vitro Digestion of Chestnut and Quebracho Tannin Extracts : Antimicrobial Effect, Antioxidant Capacity and Cytomodulatory Activity in Swine Intestinal IPEC-J2 Cells / S. Reggi, C. Giromini, M. Dell'Anno, A. Baldi, R. Rebucci, L. Rossi. - In: ANIMALS. - ISSN 2076-2615. - 10:2(2020 Jan 23), pp. 195.1-195.14.
|Titolo:||In Vitro Digestion of Chestnut and Quebracho Tannin Extracts : Antimicrobial Effect, Antioxidant Capacity and Cytomodulatory Activity in Swine Intestinal IPEC-J2 Cells|
GIROMINI, CARLOTTA (Corresponding)
|Parole Chiave:||plant extracts; tannin; in vitro digestion; growth inhibition; enterotoxigenic E. coli; antimicrobial activity; antioxidant capacity; IPEC-J2 intestinal cell|
|Settore Scientifico Disciplinare:||Settore AGR/18 - Nutrizione e Alimentazione Animale|
|Progetto:||FOODTECH Prodotti innovativi in campo zooetecnico|
|Data di pubblicazione:||23-gen-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/ani10020195|
|Appare nelle tipologie:||01 - Articolo su periodico|