We have developed biocatalytic methods for the stereoselective reduction of cyclic prochiral 1,3-diketones for the production of optically active β-hydroxyketones and/or 1,3-diols. The recombinant ketoreductase KRED1-Pglu (formulated as purified catalyst) and whole cells of wild type Escherichia coli DE3 Star were used as biocatalysts, displaying different and sometimes complementary stereoselectivity, thus allowing the preparation of stereochemically pure β-hydroxyketones (12–66% isolated yields, > 99% e.e.) and 1,3-diols (40–60% isolated yields, > 99% e.e.).
Stereoselective Reduction of Prochiral Cyclic 1,3-Diketones Using Different Biocatalysts / M.L. Contente, F. Dall'Oglio, F. Annunziata, F. Molinari, M. Rabuffetti, D. Romano, L. Tamborini, D. Rother, A. Pinto. - In: CATALYSIS LETTERS. - ISSN 1011-372X. - (2019). [Epub ahead of print]
Stereoselective Reduction of Prochiral Cyclic 1,3-Diketones Using Different Biocatalysts
M.L. ContentePrimo
;F. Dall'OglioSecondo
;F. Annunziata;F. Molinari;M. Rabuffetti;D. Romano;L. Tamborini;A. Pinto
Ultimo
2019
Abstract
We have developed biocatalytic methods for the stereoselective reduction of cyclic prochiral 1,3-diketones for the production of optically active β-hydroxyketones and/or 1,3-diols. The recombinant ketoreductase KRED1-Pglu (formulated as purified catalyst) and whole cells of wild type Escherichia coli DE3 Star were used as biocatalysts, displaying different and sometimes complementary stereoselectivity, thus allowing the preparation of stereochemically pure β-hydroxyketones (12–66% isolated yields, > 99% e.e.) and 1,3-diols (40–60% isolated yields, > 99% e.e.).File | Dimensione | Formato | |
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