Antibiotics play an important role in ensuring the health and welfare of poultry and are commonly administered to treat and prevent respiratory diseases and other microbial infections, but are often illicitly used in poultry breeding, via the drinking water or feed. Widespread antibiotic use and the antimicrobial resistance phenomenon demand new analytical methods and the use of non-conventional matrices increasingly necessary for safe food control. We present a method developed to detect six common antibiotics used in poultry breeding, in the unconventional matrix, feathers, compared to muscle and liver. The analysis for the presence of two β-lactams (penicillin V, amoxicillin), two fluoroquinolones (enrofloxacin, ciprofloxacin), one phenicol (thiamphenicol) and one macrolide (tylosin) was validated and achieved by HPLC–HRMS, with the ultimate aim to identify untargeted metabolites in broilers subjected to different therapeutic protocols. All the validated method parameters met the regulatory requirements. Muscle and liver were not effective matrices when the withdrawal periods were largely respected. Conversely, feathers proved a promising matrix for the detection of all the studied antibiotics, in the range of 8.72–1885.32 ng g−1, except penicillin V. Like other nonconventional matrices, such as teeth, the antibiotics detected in feathers existed in their unmetabolised form.
Antibiotics in food of animal origin : their role and distribution among food chain and relevance of analytical controls for food inspection / S. Panseri, M. Nobile, F. Arioli, L.M. Chiesa. ((Intervento presentato al convegno World Summit on Advancement in Food Science and Technology tenutosi a Valencia nel 2019.
Antibiotics in food of animal origin : their role and distribution among food chain and relevance of analytical controls for food inspection
S. Panseri;M. Nobile;F. Arioli;L.M. Chiesa
2019
Abstract
Antibiotics play an important role in ensuring the health and welfare of poultry and are commonly administered to treat and prevent respiratory diseases and other microbial infections, but are often illicitly used in poultry breeding, via the drinking water or feed. Widespread antibiotic use and the antimicrobial resistance phenomenon demand new analytical methods and the use of non-conventional matrices increasingly necessary for safe food control. We present a method developed to detect six common antibiotics used in poultry breeding, in the unconventional matrix, feathers, compared to muscle and liver. The analysis for the presence of two β-lactams (penicillin V, amoxicillin), two fluoroquinolones (enrofloxacin, ciprofloxacin), one phenicol (thiamphenicol) and one macrolide (tylosin) was validated and achieved by HPLC–HRMS, with the ultimate aim to identify untargeted metabolites in broilers subjected to different therapeutic protocols. All the validated method parameters met the regulatory requirements. Muscle and liver were not effective matrices when the withdrawal periods were largely respected. Conversely, feathers proved a promising matrix for the detection of all the studied antibiotics, in the range of 8.72–1885.32 ng g−1, except penicillin V. Like other nonconventional matrices, such as teeth, the antibiotics detected in feathers existed in their unmetabolised form.File | Dimensione | Formato | |
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115 FSIT- due Abstracts valencia 2019 pg 14 e pg 16.pdf
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