Many naturally available compounds as n3-polyunsaturated fatty acids (EPA: Eicosapentaenoic acidand DHA: Docosahexaenoic acid), conjugated linoleic acid, milk exosomes and plant extract from Macleaya cordata exhibits anti-inflammatory effects. From previous studies, these bioactive compounds demonstrated a multitude of beneficiary effects in both human and animal health and are considered as potential therapeutic agents with pharmaceutical properties. Due to their health benefits, new ways to incorporate them in human diet through poultry and livestock nutrition is extensively studied and therefore, it is first important to determine its anti-inflammatory effects in cell-based inflammatory models. The gastrointestinal tract (GI) is the first site where food is broken down and nutrients are absorbed and therefore the GI cell models are widely preferred for food/feed analysis. In this respect, it becomes of paramount importance to establish inflammatory cell line models of intestinal epithelia. Therefore, in the present study, we demonstrated the inflammatory response of IPEC-J2 cell lines of neonate porcine intestinal epithelium challenged against different stimuli as cell wall lipopolysaccharides (LPS) of Gramnegative bacteria as Escherichia coli and Salmonella, and chemical such as dextran sodium sulphate (DSS), analysed by MTT cell viability assay. The cells were treated with each stimulus in a dose-dependent manner (0.15–10% for DSS, 1.56–100μg/mL for LPS) for 24h and thereafter viability was measured and the concentration at 50% inhibition (IC50) was calculated using regression analysis. The IPEC-J2 cells exhibited an IC50 value of 2.89% for DSS challenge and 12.77μg/mL for Salmonella LPS. The E. coli did not show any significant inflammatory response even with the challenge of highest dose as 100μg/mL. These results suggest that the epithelial cells are specific for different biological challenge as bacterial LPS and the DSS chemical proves to be potent inflammatory agent even at small doses and can be effectively used to induce inflammatory response to study anti- nflammatory properties of food/feed additives.

Establishment of inflammatory in vitro intestinal epithelial models for translational animal nutrition / T.S. Sundaram, C. Giromini, R. Rebucci, A. Baldi. - In: ITALIAN JOURNAL OF ANIMAL SCIENCE. - ISSN 1828-051X. - 18:suppl. 1(2019), pp. 159-159. ((Intervento presentato al 23. convegno ASPA tenutosi a Sorrento nel 2019.

Establishment of inflammatory in vitro intestinal epithelial models for translational animal nutrition

T.S. Sundaram;C. Giromini;R. Rebucci;A. Baldi
2019

Abstract

Many naturally available compounds as n3-polyunsaturated fatty acids (EPA: Eicosapentaenoic acidand DHA: Docosahexaenoic acid), conjugated linoleic acid, milk exosomes and plant extract from Macleaya cordata exhibits anti-inflammatory effects. From previous studies, these bioactive compounds demonstrated a multitude of beneficiary effects in both human and animal health and are considered as potential therapeutic agents with pharmaceutical properties. Due to their health benefits, new ways to incorporate them in human diet through poultry and livestock nutrition is extensively studied and therefore, it is first important to determine its anti-inflammatory effects in cell-based inflammatory models. The gastrointestinal tract (GI) is the first site where food is broken down and nutrients are absorbed and therefore the GI cell models are widely preferred for food/feed analysis. In this respect, it becomes of paramount importance to establish inflammatory cell line models of intestinal epithelia. Therefore, in the present study, we demonstrated the inflammatory response of IPEC-J2 cell lines of neonate porcine intestinal epithelium challenged against different stimuli as cell wall lipopolysaccharides (LPS) of Gramnegative bacteria as Escherichia coli and Salmonella, and chemical such as dextran sodium sulphate (DSS), analysed by MTT cell viability assay. The cells were treated with each stimulus in a dose-dependent manner (0.15–10% for DSS, 1.56–100μg/mL for LPS) for 24h and thereafter viability was measured and the concentration at 50% inhibition (IC50) was calculated using regression analysis. The IPEC-J2 cells exhibited an IC50 value of 2.89% for DSS challenge and 12.77μg/mL for Salmonella LPS. The E. coli did not show any significant inflammatory response even with the challenge of highest dose as 100μg/mL. These results suggest that the epithelial cells are specific for different biological challenge as bacterial LPS and the DSS chemical proves to be potent inflammatory agent even at small doses and can be effectively used to induce inflammatory response to study anti- nflammatory properties of food/feed additives.
Settore AGR/18 - Nutrizione e Alimentazione Animale
Article (author)
File in questo prodotto:
File Dimensione Formato  
Sundaram et al., 2019-ASPA 23rd Congress Book of Abstracts.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 6.54 MB
Formato Adobe PDF
6.54 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/672851
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact