In the present study, we developed a microplate reader assay to detect natural products able to limit toxin production by the fungus Fusarium graminearum, the major cause of Fusarium Head Blight in cereals and trichothecene contamination in grains worldwide. In the biosensor assay, we exploited a F. graminearum isolate expressing GFP-tagged trichodiene synthase, encoded by TRI5 (TRI5-GFP), which catalyses the first step of the trichothecene biosynthesis pathway. This allowed us to monitor the first step of toxin production by fluorimetric assay. Fungal spores were treated with filtrates from streptomycete liquid cultures in order to evaluate their influence on fungal growth and toxin production, integrating absorbance and fluorescence measurements. The correlation between the fluorimetric assay based on the TRI5-GFP detection and the amount of deoxynivalenol (DON) measured with ELISA technique was assessed. The new method allowed to identify crude filtrates able to reduce toxin production as accurately as ELISA. The main advantages of the newly developed assay are the reduction of the analysis costs and lower use of chemicals and consumables. It contributes to increase the speed and capacity to screen large set of molecules and natural extracts. A historical collection of Streptomyces strains was screened by the newly developed method and five bacterial strains have been identified as producing bioactive molecules able to inhibit the fungal growth and/or the toxin synthesis (DON).

Development of a Fusarium graminearum biosensor assay to monitor the activity of naturally derived products to control trichothecene production / E.M. Colombo, A. Kunova, C. Pizzatti, M. Saracchi, P. Cortesi, D. Gardiner, M. Pasquali. ((Intervento presentato al 7. convegno Conference on Physiology of yeasts and filamentous fungi tenutosi a Milano nel 2019.

Development of a Fusarium graminearum biosensor assay to monitor the activity of naturally derived products to control trichothecene production

E.M. Colombo
Primo
;
A. Kunova
Secondo
;
C. Pizzatti;M. Saracchi;P. Cortesi;M. Pasquali
Ultimo
2019

Abstract

In the present study, we developed a microplate reader assay to detect natural products able to limit toxin production by the fungus Fusarium graminearum, the major cause of Fusarium Head Blight in cereals and trichothecene contamination in grains worldwide. In the biosensor assay, we exploited a F. graminearum isolate expressing GFP-tagged trichodiene synthase, encoded by TRI5 (TRI5-GFP), which catalyses the first step of the trichothecene biosynthesis pathway. This allowed us to monitor the first step of toxin production by fluorimetric assay. Fungal spores were treated with filtrates from streptomycete liquid cultures in order to evaluate their influence on fungal growth and toxin production, integrating absorbance and fluorescence measurements. The correlation between the fluorimetric assay based on the TRI5-GFP detection and the amount of deoxynivalenol (DON) measured with ELISA technique was assessed. The new method allowed to identify crude filtrates able to reduce toxin production as accurately as ELISA. The main advantages of the newly developed assay are the reduction of the analysis costs and lower use of chemicals and consumables. It contributes to increase the speed and capacity to screen large set of molecules and natural extracts. A historical collection of Streptomyces strains was screened by the newly developed method and five bacterial strains have been identified as producing bioactive molecules able to inhibit the fungal growth and/or the toxin synthesis (DON).
deoxynivalenol; GFP; streptomycetes
Settore AGR/12 - Patologia Vegetale
Development of a Fusarium graminearum biosensor assay to monitor the activity of naturally derived products to control trichothecene production / E.M. Colombo, A. Kunova, C. Pizzatti, M. Saracchi, P. Cortesi, D. Gardiner, M. Pasquali. ((Intervento presentato al 7. convegno Conference on Physiology of yeasts and filamentous fungi tenutosi a Milano nel 2019.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/652226
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