There are at least 554 identified compounds in C. sativa L., among them 113 phytocannabinoids and 120 terpenes. Phytocomplex composition differences between the pharmaceutical properties of different medical cannabis chemotype have been attributed to strict interactions, defined as âentourage effectâ, between cannabinoids and terpenes as a result of synergic action. The chemical complexity of its bioactive constituents highlight the need for standardised and well-defined analytical approaches able to characterise the plant chemotype, the herbal drug quality as well as to monitor the quality of pharmaceutical cannabis extracts and preparations. Hence, in the first part of this study an analytical procedures involving the combination of headspace-solid-phase microextraction (HS-SPME) coupled to GCâMS and High Resolution Mass-Spectrometry LC-HRMS (OrbitrapÂ®) were set up, validated and applied for the in-depth profiling and fingerprinting of cannabinoids and terpenes in two authorised medical grade varieties of Cannabis sativa L. inflorescences (BedrocanÂ®and BediolÂ®) and in obtained macerated oils. To better understand the trend of all volatile compounds and cannabinoids during oil storage a new procedure for cannabis macerated oil preparation without any thermal step was tested and compared with the existing conventional methods to assess the potentially detrimental effect of heating on overall product quality.
Comprehensive quality evaluation of medical Cannabis sativa L. inflorescence and macerated oils based on HS-SPME coupled to GC-MS and LC-HRMS (q-exactive orbitrap (R)) approach / C. Lorenzo, D. Pentimalli, S. Panseri, L. Giupponi, F. Gelmini, G. Beretta, D. Vitali, B. Massimo, E. Zilio, R. Pavlovic, A. Giorgi. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 150(2018 Feb 20), pp. 208-219.
|Titolo:||Comprehensive quality evaluation of medical Cannabis sativa L. inflorescence and macerated oils based on HS-SPME coupled to GC-MS and LC-HRMS (q-exactive orbitrap (R)) approach|
PANSERI, SARA (Corresponding)
|Settore Scientifico Disciplinare:||Settore BIO/03 - Botanica Ambientale e Applicata|
|Data di pubblicazione:||20-feb-2018|
|Data ahead of print / Data di stampa:||22-dic-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.jpba.2017.11.073|
|Appare nelle tipologie:||01 - Articolo su periodico|