A follow-up of antibiotics (tetracyclines, fluoroquinolones, cephalosporins, penicillins and amphenicols) in the bovine urine is important for two reasons: to understand if they are still present in organism, and whether their occurrence in urine might be considered as an environmental risk. A validated HPLC–MS/MS method (Decision 2002/657/EC) for antibiotics determination in bovine urine was developed. CCα and CCβ were in the range of 0.58–0.83 and 0.55–1.1 ng mL−1, respectively. Recoveries were 92–108%, with inter-day repeatability below 12%. Analysis of bovine urine revealed frequent presence of tetracyclines, which was related with animal’s age. The cause, most presumably, might be found in different therapeutic protocols applied for veal calves and young bulls enrolled in this study. Most abundant was oxytetracycline with highest level in veal calves (1718 ng mL−1) vs. young bulls (2.8 ng mL−1). Our results indicate the necessity of antibiotics monitoring in bovine urine before animals undergo further processing in the food industry.

Determination of veterinary antibiotics in bovine urine by liquid chromatography-tandem mass spectrometry / L. Chiesa, M. Nobile, F. Arioli, D. Britti, N. Trutic, R. Pavlovic, S. Panseri. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 185(2015 Oct 15), pp. 7-15.

Determination of veterinary antibiotics in bovine urine by liquid chromatography-tandem mass spectrometry

L. Chiesa
Primo
;
M. Nobile
Secondo
;
F. Arioli
;
R. Pavlovic
Penultimo
;
S. Panseri
Ultimo
2015-10-15

Abstract

A follow-up of antibiotics (tetracyclines, fluoroquinolones, cephalosporins, penicillins and amphenicols) in the bovine urine is important for two reasons: to understand if they are still present in organism, and whether their occurrence in urine might be considered as an environmental risk. A validated HPLC–MS/MS method (Decision 2002/657/EC) for antibiotics determination in bovine urine was developed. CCα and CCβ were in the range of 0.58–0.83 and 0.55–1.1 ng mL−1, respectively. Recoveries were 92–108%, with inter-day repeatability below 12%. Analysis of bovine urine revealed frequent presence of tetracyclines, which was related with animal’s age. The cause, most presumably, might be found in different therapeutic protocols applied for veal calves and young bulls enrolled in this study. Most abundant was oxytetracycline with highest level in veal calves (1718 ng mL−1) vs. young bulls (2.8 ng mL−1). Our results indicate the necessity of antibiotics monitoring in bovine urine before animals undergo further processing in the food industry.
Amphenicols; Bovine urine; Cephalosporins; LC-MS/MS; Penicillins; Tetracyclines-Fluoroquinolones; Food Science; Analytical Chemistry
Settore VET/07 - Farmacologia e Tossicologia Veterinaria
Settore VET/04 - Ispezione degli Alimenti di Origine Animale
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/340799
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