Cyclic voltammetry has been applied to the rapid measurement of free SH-compounds in food flours samples. The protocol is based on the electrochemical adaptation of the Ellman's test, where the DTNB reacts with SH-compounds present in food flour extracts, resulting in the equimolar production of the electroactive compound TNBA which, in turns, reacts with phenylendiamine. The reaction is found to provide an analytical signal from which to quantify indirectly free sulfhydryl compounds in flours extracts.
Voltammetric Detection of Free Sulfhydryl Compounds in Food Flours / M. Scampicchio, N.S. Lawrence, A. Arecchi, S. Cosio, S. Mannino. - In: ELECTROANALYSIS. - ISSN 1040-0397. - 19:1(2007), pp. 85-90. [10.1002/elan.200603709]
Voltammetric Detection of Free Sulfhydryl Compounds in Food Flours
M. ScampicchioPrimo
;A. Arecchi;S. CosioPenultimo
;S. ManninoUltimo
2007
Abstract
Cyclic voltammetry has been applied to the rapid measurement of free SH-compounds in food flours samples. The protocol is based on the electrochemical adaptation of the Ellman's test, where the DTNB reacts with SH-compounds present in food flour extracts, resulting in the equimolar production of the electroactive compound TNBA which, in turns, reacts with phenylendiamine. The reaction is found to provide an analytical signal from which to quantify indirectly free sulfhydryl compounds in flours extracts.
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