During hydrolysis of bovine milk caseins, lactic acid bacteria may be able to release peptides that possess immunomodulatory activity. The bacterial capability to digest caseins can be attributed to the presence of cell envelope-associated proteinases (CEPs). In this study, sodium caseinate from unprocessed bovine milk was submitted to the hydrolysis with CEPs of lactic acid bacterial strains. The hydrolysates were tested for their immunomodularoty activity in general, and for anti-inflammatory activity in particular. To evaluate the immunomodulatory activity of hydrolysates on human intestinal tissue, a human colorectal adenocarcinoma cell line Caco-2, transfected with nuclear factor kappa B (NF-kB)-inducible luciferase reporter plasmid, was used as a model system. Sodium caseinate hydrolysate, produced after digestion with CEP of Lactococcus lactis subsp. lactis GR5 did not show influence on basal NF-B activity, but its 3 kDa-separated fraction significantly decreased the basal NF-kB activity both, in the absence, and in the presence of pro-inflammatory stimulus of interleukin 1 beta, showing an anti-inflammatory activity in the latter case.

Immunomodulatory activity of casein hydrolysates produced after digestion with lactic acid bacterial cell envelope-associated proteinases / M. Stuknyte, I. De Noni, S. Guglielmetti, M. Minuzzo, D. Mora. ((Intervento presentato al convegno Congresso Cortona Procarioti tenutosi a Cortona nel 2010.

Immunomodulatory activity of casein hydrolysates produced after digestion with lactic acid bacterial cell envelope-associated proteinases

M. Stuknyte
Primo
;
I. De Noni
Secondo
;
S. Guglielmetti;M. Minuzzo
Penultimo
;
D. Mora
Ultimo
2010

Abstract

During hydrolysis of bovine milk caseins, lactic acid bacteria may be able to release peptides that possess immunomodulatory activity. The bacterial capability to digest caseins can be attributed to the presence of cell envelope-associated proteinases (CEPs). In this study, sodium caseinate from unprocessed bovine milk was submitted to the hydrolysis with CEPs of lactic acid bacterial strains. The hydrolysates were tested for their immunomodularoty activity in general, and for anti-inflammatory activity in particular. To evaluate the immunomodulatory activity of hydrolysates on human intestinal tissue, a human colorectal adenocarcinoma cell line Caco-2, transfected with nuclear factor kappa B (NF-kB)-inducible luciferase reporter plasmid, was used as a model system. Sodium caseinate hydrolysate, produced after digestion with CEP of Lactococcus lactis subsp. lactis GR5 did not show influence on basal NF-B activity, but its 3 kDa-separated fraction significantly decreased the basal NF-kB activity both, in the absence, and in the presence of pro-inflammatory stimulus of interleukin 1 beta, showing an anti-inflammatory activity in the latter case.
Settore AGR/15 - Scienze e Tecnologie Alimentari
Settore AGR/16 - Microbiologia Agraria
Settore BIO/10 - Biochimica
Settore BIO/11 - Biologia Molecolare
Società Italiana di Microbiologia Generale e Biotecnologie Microbiche (SIMGBM)
http://www.simgbm.it/index.php?option=com_content&view=article&id=74%3Acongresso-cortona-procarioti-14-15-aprile-2010&Itemid=11
Immunomodulatory activity of casein hydrolysates produced after digestion with lactic acid bacterial cell envelope-associated proteinases / M. Stuknyte, I. De Noni, S. Guglielmetti, M. Minuzzo, D. Mora. ((Intervento presentato al convegno Congresso Cortona Procarioti tenutosi a Cortona nel 2010.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/254649
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