Candida milleri, together with Candida humilis, is the most representative yeast species found in type I sourdough ecosystems. In this work, comparison of the ITS region and the D1/D2 domain of 26S rDNA gene partial sequences, karyotyping, mtDNA-RFLP analysis, Intron Splice Site dispersion (ISS-PCR) and (GTG)5 microsatellite analyses, assimilation test of different carbohydrates, and metabolome assessment by FT-IR analysis, were investigated in seventeen strains isolated from four different companies as well as in type strains CBS6897T and CBS5658T. Most isolates were ascribed to C.milleri, even if a strong relatedness was confirmed with C.humilis as well, particularly for three strains. Genetic characterization showed a high degree of intraspecific polymorphism since 12 different genotypes were discriminated. The number of chromosomes varied from 9 to 13 and their size ranged from less than 0.3 to over 2Mbp. Phenotypic traits let to recognize 9 different profiles of carbon sources assimilation. FT-IR spectra from yeast cells cultivated in different media and collected at different growth phases revealed a diversity of behaviour among strains in accordance with the results of PCR-based fingerprinting. A clear evidence of the polymorphic status of C.milleri species is provided thus representing an important feature for the development of technological applications in bakery industries.

Candida milleri species reveals intraspecific genetic and metabolic polymorphisms / I. Vigentini, D. Antoniani, L. Roscini, A. Comasio, S. Galafassi, C. Picozzi, L. Corte, C. Compagno, F.D. Bello, G. Cardinali, R. Foschino. - In: FOOD MICROBIOLOGY. - ISSN 0740-0020. - 42(2014), pp. 72-81. [10.1016/j.fm.2014.02.011]

Candida milleri species reveals intraspecific genetic and metabolic polymorphisms

I. Vigentini
Primo
;
D. Antoniani
Secondo
;
S. Galafassi;C. Picozzi;C. Compagno;R. Foschino
Ultimo
2014

Abstract

Candida milleri, together with Candida humilis, is the most representative yeast species found in type I sourdough ecosystems. In this work, comparison of the ITS region and the D1/D2 domain of 26S rDNA gene partial sequences, karyotyping, mtDNA-RFLP analysis, Intron Splice Site dispersion (ISS-PCR) and (GTG)5 microsatellite analyses, assimilation test of different carbohydrates, and metabolome assessment by FT-IR analysis, were investigated in seventeen strains isolated from four different companies as well as in type strains CBS6897T and CBS5658T. Most isolates were ascribed to C.milleri, even if a strong relatedness was confirmed with C.humilis as well, particularly for three strains. Genetic characterization showed a high degree of intraspecific polymorphism since 12 different genotypes were discriminated. The number of chromosomes varied from 9 to 13 and their size ranged from less than 0.3 to over 2Mbp. Phenotypic traits let to recognize 9 different profiles of carbon sources assimilation. FT-IR spectra from yeast cells cultivated in different media and collected at different growth phases revealed a diversity of behaviour among strains in accordance with the results of PCR-based fingerprinting. A clear evidence of the polymorphic status of C.milleri species is provided thus representing an important feature for the development of technological applications in bakery industries.
Candida humilis; Candida milleri; FT-IR; Sourdough; Strain typing; Yeasts
Settore AGR/16 - Microbiologia Agraria
Settore CHIM/11 - Chimica e Biotecnologia delle Fermentazioni
Article (author)
File in questo prodotto:
File Dimensione Formato  
Vigentini_et_al 2014 Food Microbiol.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 1.07 MB
Formato Adobe PDF
1.07 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/232150
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 20
  • ???jsp.display-item.citation.isi??? 19
social impact