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Transcriptomic profiling and functional assessment of omega-3 fatty acids in porcine enterocytes / T.S. Sundaram, C. Giromini, R. Rebucci, A. Baldi, M. Bhide, J. Pistl - In: Book of Abstracts of the 72nd Annual Meeting of the European Federation of Animal Science[s.l] : Wageningen Academic, 2021. - ISBN 978-90-8686-366-2. - pp. 629-629 (( Intervento presentato al 72. convegno Annual Meeting of the European Federation of Animal Science (EAAP) tenutosi a Davos nel 2021.

Transcriptomic profiling and functional assessment of omega-3 fatty acids in porcine enterocytes

T.S. Sundaram
Primo
;C. Giromini
Secondo
;R. Rebucci;A. Baldi;
2021
Marine and plant-based omega-3 polyunsaturated fatty acids (ω-3 PUFAs) are widely incorporated in animal diet to improve growth and immunity. Especially, ω-3 PUFAs such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are known to exhibit strong immunomodulatory effects as anti-inflammation and anti-oxidation. However, its molecular activity on intestinal epithelium under inflammatory and oxidative stress is not fully understood. Presently, we evaluated the dose-response, anti-inflammatory and anti-oxidative effects of EPA and DHA against lipopolysaccharides (LPS) challenge in a non-transformed porcine enterocyte model, IPEC-J2. The results showed 24 h treatment with EPA or DHA exhibited proliferative effects in IPEC-J2 cells at concentrations of 6.25-50 μM (P<0.05). Further, 24 h pre-treatment of DHA (3.3 µM), EPA (6.7 µM) or DHA:EPA (1:2; 10 µM) increased the mitochondrial activity, decreased apoptotic caspase-3/7 release by two-fold post-LPS (24 h) challenge (P<0.05). For the first time, we demonstrated the proliferative and cytoprotective properties of EPA/DHA at low concentrations in IPEC-J2 cells. Increased intracellular mitochondrial activity by ω-3 PUFAs can play a crucial role in preventing enterocyte apoptosis during inflammatory and oxidative stress. Further, to identify the novel molecular pathway of ω-3 PUFAs activity, the gene expression was evaluated by high-throughput transcriptomics technique. cDNA library was constructed and sequenced by Illumina NextSeq. The sequences were further processed and aligned to reference pig genome. Around 293 common and 149 unique differentially expressed genes corresponding to LPS challenge with and without ω-3 PUFAs pre-treatment was identified. Thus, the present outcomes highlight ω-3 PUFA mediated cellular mechanisms underpinning their function in pig nutrition.
Settore AGR/18 - Nutrizione e Alimentazione Animale
2021
Book Part (author)
03 - Contributo in volume
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/1075252
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