BC is a nutraceutical that can modulate intestinal microbiota. This study investigates theeffects of BC diet supplementation on luminal and mucosa-associated microbiota in the jejunum,caecum, and colon of rabbits. Twenty-one New Zealand White female rabbits were divided intothree experimental groups (n = 7) receiving a commercial feed (CTRL group) and the same dietsupplemented with 2.5% and 5% BC (2.5% BC and 5% BC groups, respectively), from 35 (weaning) to90 days of age (slaughtering). At slaughter, the digestive tract was removed from each animal, thenboth content and mucosa-associated microbiota of jejunum, caecum, and colon were collected andanalysed by Next Generation 16SrRNA Gene Sequencing. Significant differences were found in themicrobial composition of the three groups (i.e., beta-diversity: p < 0.01), especially in the caecum andcolon of the 2.5% BC group. The relative abundance analysis showed that the families most affectedby the BC administration were Clostridia UCG-014, Barnesiellaceae, and Eggerthellaceae. A trendwas also found for Lachnospiraceae, Akkermansiaceae, and Bacteroidaceae. A functional prediction as revealed several altered pathways in BC groups, with particular reference to amino acids andlactose metabolism. Firmicutes:Bacteroidetes ratio decreased in caecum luminal samples of the 2.5¼ group. These findings suggest that BC supplementation could positively affect the intestinalmicrobiota. However, further research is needed to establish the optimal administration dose.

Bovine Colostrum Supplementation Modulates the Intestinal Microbial Community in Rabbits / S. Agradi, P. Cremonesi, L. Menchetti, C.M. Balzaretti, M. Severgnini, F. Riva, B. Castiglioni, S. Draghi, A. DI GIANCAMILLO, M. Castrica, D. Vigo, S.C.B. Modina, V. Serra, A. Quattrone, E. Angelucci, G. Pastorelli, G. Curone, G. Brecchia. - In: ANIMALS. - ISSN 2076-2615. - 13:6(2023 Mar 08), pp. 976.1-976.19. [10.3390/ani13060976]

Bovine Colostrum Supplementation Modulates the Intestinal Microbial Community in Rabbits

S. Agradi
Primo
;
C.M. Balzaretti;F. Riva;S. Draghi;A. DI GIANCAMILLO;M. Castrica;D. Vigo;S.C.B. Modina;V. Serra;G. Pastorelli;G. Curone
Co-ultimo
;
G. Brecchia
Co-ultimo
2023

Abstract

BC is a nutraceutical that can modulate intestinal microbiota. This study investigates theeffects of BC diet supplementation on luminal and mucosa-associated microbiota in the jejunum,caecum, and colon of rabbits. Twenty-one New Zealand White female rabbits were divided intothree experimental groups (n = 7) receiving a commercial feed (CTRL group) and the same dietsupplemented with 2.5% and 5% BC (2.5% BC and 5% BC groups, respectively), from 35 (weaning) to90 days of age (slaughtering). At slaughter, the digestive tract was removed from each animal, thenboth content and mucosa-associated microbiota of jejunum, caecum, and colon were collected andanalysed by Next Generation 16SrRNA Gene Sequencing. Significant differences were found in themicrobial composition of the three groups (i.e., beta-diversity: p < 0.01), especially in the caecum andcolon of the 2.5% BC group. The relative abundance analysis showed that the families most affectedby the BC administration were Clostridia UCG-014, Barnesiellaceae, and Eggerthellaceae. A trendwas also found for Lachnospiraceae, Akkermansiaceae, and Bacteroidaceae. A functional prediction as revealed several altered pathways in BC groups, with particular reference to amino acids andlactose metabolism. Firmicutes:Bacteroidetes ratio decreased in caecum luminal samples of the 2.5¼ group. These findings suggest that BC supplementation could positively affect the intestinalmicrobiota. However, further research is needed to establish the optimal administration dose.
microbiota; caecum; nutraceutical; Firmicutes; Bacteroidetes
Settore VET/02 - Fisiologia Veterinaria
Settore VET/05 - Malattie Infettive degli Animali Domestici
Settore VET/01 - Anatomia degli Animali Domestici
Settore AGR/18 - Nutrizione e Alimentazione Animale
Settore VET/04 - Ispezione degli Alimenti di Origine Animale
Settore VET/07 - Farmacologia e Tossicologia Veterinaria
8-mar-2023
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/957552
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