A multidisciplinary investigation on Achillea moschata Wulfen (Asteraceae) is outlined herein. This work, part of the European Interreg Italy–Switzerland B-ICE project, originated from an ethnobotanical survey performed in Chiesa in Valmalenco (Sondrio, Lombardy, Northern Italy) in 2019–2021 which highlighted this species’ relevance of use in folk medicine to treat gastrointestinal diseases. In addition, this contribution included analyses of the: (a) phytochemical profile of the aqueous and methanolic extracts of the dried flower heads using LC-MS/MS; (b) morpho-anatomy and histochemistry of the vegetative and reproductive organs through Light, Fluorescence, and Scanning Electron Microscopy; (c) biological activity of the aqueous extract concerning the antioxidant and anti-inflammatory potential through cell-based in vitro models. A total of 31 compounds (5 phenolic acids, 13 flavonols, and 13 flavones) were detected, 28 of which included in both extracts. Covering and secreting trichomes were observed: the biseriate 10-celled glandular trichomes prevailing on the inflorescences represented the main sites of synthesis of the polyphenols and flavonoids detected in the extracts, along with volatile terpenoids. Finally, significant antioxidant and anti-inflammatory activities of the aqueous extract were documented, even at very low concentrations; for the first time, the in vitro tests allowed us to formulate hypotheses about the mechanism of action. This work brings an element of novelty due to the faithful reproduction of the traditional aqueous preparation and the combination of phytochemical and micromorphological research approaches.

Achillea moschata Wulfen: From Ethnobotany to Phytochemistry, Morphology, and Biological Activity / M. Bottoni, G. Baron, F. Gado, F. Milani, L. Santagostini, L. Colombo, P. Sira Colombo, E. Caporali, A. Spada, M. Biagi, C. Giuliani, P. Bruschi, G. Aldini, G. Fico. - In: MOLECULES. - ISSN 1420-3049. - 27:23(2022 Dec), pp. 8318.1-8318.21. [10.3390/molecules27238318]

Achillea moschata Wulfen: From Ethnobotany to Phytochemistry, Morphology, and Biological Activity

M. Bottoni
Primo
;
G. Baron
Secondo
;
F. Gado;F. Milani;L. Santagostini;E. Caporali;A. Spada;C. Giuliani
;
G. Aldini
Penultimo
;
G. Fico
Ultimo
2022

Abstract

A multidisciplinary investigation on Achillea moschata Wulfen (Asteraceae) is outlined herein. This work, part of the European Interreg Italy–Switzerland B-ICE project, originated from an ethnobotanical survey performed in Chiesa in Valmalenco (Sondrio, Lombardy, Northern Italy) in 2019–2021 which highlighted this species’ relevance of use in folk medicine to treat gastrointestinal diseases. In addition, this contribution included analyses of the: (a) phytochemical profile of the aqueous and methanolic extracts of the dried flower heads using LC-MS/MS; (b) morpho-anatomy and histochemistry of the vegetative and reproductive organs through Light, Fluorescence, and Scanning Electron Microscopy; (c) biological activity of the aqueous extract concerning the antioxidant and anti-inflammatory potential through cell-based in vitro models. A total of 31 compounds (5 phenolic acids, 13 flavonols, and 13 flavones) were detected, 28 of which included in both extracts. Covering and secreting trichomes were observed: the biseriate 10-celled glandular trichomes prevailing on the inflorescences represented the main sites of synthesis of the polyphenols and flavonoids detected in the extracts, along with volatile terpenoids. Finally, significant antioxidant and anti-inflammatory activities of the aqueous extract were documented, even at very low concentrations; for the first time, the in vitro tests allowed us to formulate hypotheses about the mechanism of action. This work brings an element of novelty due to the faithful reproduction of the traditional aqueous preparation and the combination of phytochemical and micromorphological research approaches.
primary data; traditional decoction; flower heads; aqueous and methanolic extracts; mass spectrometry; antioxidant activity; anti-inflammatory activity; glandular indumentum; microscopy;
Settore BIO/15 - Biologia Farmaceutica
Settore CHIM/08 - Chimica Farmaceutica
INTERREG19GFICO_01 - Bernina Terra Glacialis. Studio e valorizzazione di un patrimonio naturale e culturale di particolare pregio in una regione alpina aperta, con approcci innovativi rivolti al futuro (Bi-ICE) - FICO, GELSOMINA - Interreg Europe - 2019
23-nov-2022
https://www.mdpi.com/1420-3049/27/23/8318
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/947031
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