In the mature central nervous system (CNS), oligodendrocytes (OLs) provide support and insulation to axons thanks to the production of a myelin sheath. During their maturation to myelinating cells, OLs require energy and building blocks for lipids, which implies a great investment of energy fuels and molecular sources of carbon. The oligodendroglial G protein-coupled receptor 17 (GPR17) has emerged as a key player in OL maturation; it reaches maximal expression in pre-OLs, but then it has to be internalized to allow terminal maturation. In this study, we aim at elucidating the role of physiological GPR17 downregulation in OL metabolism by applying transcriptomics, metabolomics and lipidomics on differentiating OLs. After GPR17 silencing, we found a significant increase in mature OL markers and alteration of several genes involved in glucose metabolism and lipid biosynthesis. We also observed an increased release of lactate, which is partially responsible for the maturation boost induced by GPR17 downregulation. Concomitantly, GPR17 depletion also changed the kinetics of specific myelin lipid classes. Globally, this study unveils a functional link between GPR17 expression, lactate release and myelin composition, and suggests that innovative interventions targeting GPR17 may help to foster endogenous myelination in demyelinating diseases.

Rewiring of Glucose and Lipid Metabolism Induced by G Protein-Coupled Receptor 17 Silencing Enables the Transition of Oligodendrocyte Progenitors to Myelinating Cells / D. Marangon, M. Audano, S. Pedretti, M. Fumagalli, N. Mitro, D. Lecca, D. Caruso, M.P. Abbracchio. - In: CELLS. - ISSN 2073-4409. - 11:15(2022 Aug 02), pp. 2369.1-2369.20. [10.3390/cells11152369]

Rewiring of Glucose and Lipid Metabolism Induced by G Protein-Coupled Receptor 17 Silencing Enables the Transition of Oligodendrocyte Progenitors to Myelinating Cells

D. Marangon
Primo
;
M. Audano
Secondo
;
S. Pedretti;M. Fumagalli;N. Mitro;D. Lecca;D. Caruso
Penultimo
;
M.P. Abbracchio
Ultimo
2022-08-02

Abstract

In the mature central nervous system (CNS), oligodendrocytes (OLs) provide support and insulation to axons thanks to the production of a myelin sheath. During their maturation to myelinating cells, OLs require energy and building blocks for lipids, which implies a great investment of energy fuels and molecular sources of carbon. The oligodendroglial G protein-coupled receptor 17 (GPR17) has emerged as a key player in OL maturation; it reaches maximal expression in pre-OLs, but then it has to be internalized to allow terminal maturation. In this study, we aim at elucidating the role of physiological GPR17 downregulation in OL metabolism by applying transcriptomics, metabolomics and lipidomics on differentiating OLs. After GPR17 silencing, we found a significant increase in mature OL markers and alteration of several genes involved in glucose metabolism and lipid biosynthesis. We also observed an increased release of lactate, which is partially responsible for the maturation boost induced by GPR17 downregulation. Concomitantly, GPR17 depletion also changed the kinetics of specific myelin lipid classes. Globally, this study unveils a functional link between GPR17 expression, lactate release and myelin composition, and suggests that innovative interventions targeting GPR17 may help to foster endogenous myelination in demyelinating diseases.
energy metabolism; glycolysis; lactate; lipidomics; metabolomics; myelin lipids; myelination; oligodendrocyte; oligodendrocyte progenitor cell; transcriptomics; Cell Differentiation; Glucose; Lactates; Lipid Metabolism; Lipids; Nerve Tissue Proteins; Receptors, G-Protein-Coupled; Oligodendrocyte Precursor Cells
Settore BIO/14 - Farmacologia
https://www.mdpi.com/2073-4409/11/15/2369
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/936770
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