The Eurasian grapevine (Vitis vinifera), a species cultivated worldwide for high-quality wine production, is extremely susceptible to the agent of downy mildew, Plasmopara viticola. Nevertheless, germplasm from Georgia (Southern Caucasus, the first grapevine domestication centre), characterized by a high genetic variability, showed resistance traits to P. viticola. The cultivar Mgaloblishvili exhibited the most promising phenotype in terms of resistance against P. viticola. Its defence response results in: i) low disease intensity; ii) low sporulation; iii) damaged mycelium; iv) production of antimicrobial compounds such as volatile organic compounds (VOCs), whose effectiveness on the pathogen was evaluated by leafdisc assays. At the transcriptomic level, its resistance mechanism is determined by the differential expression of both resistance and susceptible genes. The resistance genes are related to: i) pathogen recognition through PAMP, DAMP and effector receptors; ii) ethylene signalling pathway; iii) synthesis of antimicrobial compounds (VOCs) and fungal wall degrading enzymes; iv) development of structural barriers (cell wall reinforcement). The first putative susceptible gene was the transcription factor VviLBDIf7 gene, whose validation was carried out by dsRNA (double-stranded RNA) assay. In this work, these unique results on plant-pathogen interaction are reviewed with the aim of developing new strategies to control the disease.

Dissecting the susceptibility/resistance mechanism of Vitis vinifera for the future control of downy mildew / V. Ricciardi, D. Marcianò, M. Sargolzaei, E. Marrone Fassolo, D. Fracassetti, M. Brilli, M. Moser, S.J. Vahid, E. Tavakole, G. Maddalena, A. Passera, P. Casati, M. Pindo, A. Cestaro, A. Costa, M.C. Bonza, D. Maghradze, A. Tirelli, O. Failla, P.A. Bianco, F. Quaglino, S.L. Toffolatti, G. De Lorenzis. - In: BIO WEB OF CONFERENCES. - ISSN 2117-4458. - 44:(2022 Jan 31), pp. 04002.1-04002.5. ((Intervento presentato al 8. convegno CO.NA.VI. 2020 Convegno Nazionale di Viticoltura tenutosi a Udine nel 2021 [10.1051/bioconf/20224404002].

Dissecting the susceptibility/resistance mechanism of Vitis vinifera for the future control of downy mildew

V. Ricciardi
Primo
;
M. Sargolzaei;D. Fracassetti;M. Brilli;G. Maddalena;A. Passera;P. Casati;A. Cestaro;A. Costa;M.C. Bonza;A. Tirelli;O. Failla;P.A. Bianco;F. Quaglino;S.L. Toffolatti
Penultimo
;
G. De Lorenzis
Ultimo
2022-01-31

Abstract

The Eurasian grapevine (Vitis vinifera), a species cultivated worldwide for high-quality wine production, is extremely susceptible to the agent of downy mildew, Plasmopara viticola. Nevertheless, germplasm from Georgia (Southern Caucasus, the first grapevine domestication centre), characterized by a high genetic variability, showed resistance traits to P. viticola. The cultivar Mgaloblishvili exhibited the most promising phenotype in terms of resistance against P. viticola. Its defence response results in: i) low disease intensity; ii) low sporulation; iii) damaged mycelium; iv) production of antimicrobial compounds such as volatile organic compounds (VOCs), whose effectiveness on the pathogen was evaluated by leafdisc assays. At the transcriptomic level, its resistance mechanism is determined by the differential expression of both resistance and susceptible genes. The resistance genes are related to: i) pathogen recognition through PAMP, DAMP and effector receptors; ii) ethylene signalling pathway; iii) synthesis of antimicrobial compounds (VOCs) and fungal wall degrading enzymes; iv) development of structural barriers (cell wall reinforcement). The first putative susceptible gene was the transcription factor VviLBDIf7 gene, whose validation was carried out by dsRNA (double-stranded RNA) assay. In this work, these unique results on plant-pathogen interaction are reviewed with the aim of developing new strategies to control the disease.
Settore AGR/12 - Patologia Vegetale
Settore AGR/03 - Arboricoltura Generale e Coltivazioni Arboree
https://www.bio-conferences.org/articles/bioconf/abs/2022/03/bioconf_conavi2022_04002/bioconf_conavi2022_04002.html
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/932047
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