In vivo cell reprogramming of glial cells offers a promising way to generate new neurons in the adult mammalian nervous system. This approach might compensate for neuronal loss occur-ring in neurological disorders, but clinically viable tools are needed to advance this strategy from bench to bedside. Recently published work has described the successful neuronal conversion of glial cells through the repression of a single gene, polypyrimidine tract‐binding protein 1 (Ptbp1), which encodes a key RNA‐binding protein. Newly converted neurons not only express correct markers but they also functionally integrate into endogenous brain circuits and modify disease symptoms in in vivo models of neurodegenerative diseases. However, doubts about the nature of “converted” neurons, in particular in vivo, have been raised, based on concerns about tracking reporter genes in converted cells. More robust lineage tracing is needed to draw definitive conclusions about the re-liability of this strategy. In vivo reprogramming and the possibility of implementing it with approaches that could be translated into the clinic with antisense oligonucleotides targeting a single gene like Ptbp1 are hot topics. They warrant further investigation with stringent methods and crite-ria of evaluation for the ultimate treatment of neurological diseases.
Targeting PTB for Glia‐to‐Neuron Reprogramming In Vitro and In Vivo for Therapeutic Development in Neurological Diseases / M. Contardo, R. De Gioia, D. Gagliardi, G.P. Comi, L. Ottoboni, M. Nizzardo, S. Corti. - In: BIOMEDICINES. - ISSN 2227-9059. - 10:2(2022 Feb 07), pp. 399.1-399.12. [10.3390/biomedicines10020399]
Targeting PTB for Glia‐to‐Neuron Reprogramming In Vitro and In Vivo for Therapeutic Development in Neurological Diseases
D. GagliardiSecondo
Writing – Original Draft Preparation
;G.P. Comi;L. Ottoboni;M. NizzardoPenultimo
;S. Corti
Ultimo
2022
Abstract
In vivo cell reprogramming of glial cells offers a promising way to generate new neurons in the adult mammalian nervous system. This approach might compensate for neuronal loss occur-ring in neurological disorders, but clinically viable tools are needed to advance this strategy from bench to bedside. Recently published work has described the successful neuronal conversion of glial cells through the repression of a single gene, polypyrimidine tract‐binding protein 1 (Ptbp1), which encodes a key RNA‐binding protein. Newly converted neurons not only express correct markers but they also functionally integrate into endogenous brain circuits and modify disease symptoms in in vivo models of neurodegenerative diseases. However, doubts about the nature of “converted” neurons, in particular in vivo, have been raised, based on concerns about tracking reporter genes in converted cells. More robust lineage tracing is needed to draw definitive conclusions about the re-liability of this strategy. In vivo reprogramming and the possibility of implementing it with approaches that could be translated into the clinic with antisense oligonucleotides targeting a single gene like Ptbp1 are hot topics. They warrant further investigation with stringent methods and crite-ria of evaluation for the ultimate treatment of neurological diseases.File | Dimensione | Formato | |
---|---|---|---|
Targeting PTB for Glia-to-Neuron Reprogramming In Vitro and In Vivo for Therapeutic Development in Neurological Diseases.pdf
accesso aperto
Tipologia:
Publisher's version/PDF
Dimensione
546.68 kB
Formato
Adobe PDF
|
546.68 kB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.