Achieving efficient distribution of neural stem cells throughout the central nervous system (CNS) and robust generation of specific neurons is a major challenge for the development of cell-mediated therapy for neurodegenerative diseases. We isolated a primitive neural stem cell subset, double positive for LeX(Le) and CXCR4(CX) antigens that possesses CNS homing potential and extensive neuronal repopulating capacity. Le(+)CX(+) cells are multipotential and can generate neurons as well as myogenic and endothelial cells. In vivo Le(+)CX(+) cells displayed widespread incorporation and differentiated into cortical and hippocampal pyramidal neurons. Since intravenous delivery could be a less invasive route of transplantation, we investigated whether Le(+)CX(+) cells could migrate across endothelial monolayers. Intracerebral coadministration of SDF enabled migration of intravenously injected Le(+) CX+ cells into the CNS and a small, yet significant, number of donor cells differentiated into neurons. The isolation of a specific neural stem cell population could offer major advantages to neuronal replacement strategies.

Multipotentiality, homing properties and pyramidal neurogenesis of CNS-derived LeX(ssea-1)+/CXCR4+ stem cells / S. Corti, F. Locatelli, D. Papadimitriou, C. Donadoni, R. Del Bo, F. Fortunato, S. Strazzer, S. Salani, N. Bresolin, G.P. Comi. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - 19:13(2005), pp. 1860-1862.

Multipotentiality, homing properties and pyramidal neurogenesis of CNS-derived LeX(ssea-1)+/CXCR4+ stem cells

S. Corti
Primo
;
F. Locatelli
Secondo
;
R. Del Bo;F. Fortunato;N. Bresolin
Penultimo
;
G.P. Comi
2005

Abstract

Achieving efficient distribution of neural stem cells throughout the central nervous system (CNS) and robust generation of specific neurons is a major challenge for the development of cell-mediated therapy for neurodegenerative diseases. We isolated a primitive neural stem cell subset, double positive for LeX(Le) and CXCR4(CX) antigens that possesses CNS homing potential and extensive neuronal repopulating capacity. Le(+)CX(+) cells are multipotential and can generate neurons as well as myogenic and endothelial cells. In vivo Le(+)CX(+) cells displayed widespread incorporation and differentiated into cortical and hippocampal pyramidal neurons. Since intravenous delivery could be a less invasive route of transplantation, we investigated whether Le(+)CX(+) cells could migrate across endothelial monolayers. Intracerebral coadministration of SDF enabled migration of intravenously injected Le(+) CX+ cells into the CNS and a small, yet significant, number of donor cells differentiated into neurons. The isolation of a specific neural stem cell population could offer major advantages to neuronal replacement strategies.
neural stem cells; transplantation; neurodegenerative diseases
Settore MED/26 - Neurologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/9140
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