Achieving efficient distribution of neural stem cells throughout the central nervous system (CNS) and robust generation of specific neurons is a major challenge for the development of cell-mediated therapy for neurodegenerative diseases. We isolated a primitive neural stem cell subset, double positive for LeX(Le) and CXCR4(CX) antigens that possesses CNS homing potential and extensive neuronal repopulating capacity. Le(+)CX(+) cells are multipotential and can generate neurons as well as myogenic and endothelial cells. In vivo Le(+)CX(+) cells displayed widespread incorporation and differentiated into cortical and hippocampal pyramidal neurons. Since intravenous delivery could be a less invasive route of transplantation, we investigated whether Le(+)CX(+) cells could migrate across endothelial monolayers. Intracerebral coadministration of SDF enabled migration of intravenously injected Le(+) CX+ cells into the CNS and a small, yet significant, number of donor cells differentiated into neurons. The isolation of a specific neural stem cell population could offer major advantages to neuronal replacement strategies.
Multipotentiality, homing properties and pyramidal neurogenesis of CNS-derived LeX(ssea-1)+/CXCR4+ stem cells / S. Corti, F. Locatelli, D. Papadimitriou, C. Donadoni, R. Del Bo, F. Fortunato, S. Strazzer, S. Salani, N. Bresolin, G.P. Comi. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - 19:13(2005), pp. 1860-1862.
|Titolo:||Multipotentiality, homing properties and pyramidal neurogenesis of CNS-derived LeX(ssea-1)+/CXCR4+ stem cells|
CORTI, STEFANIA PAOLA (Primo)
LOCATELLI, FEDERICA (Secondo)
BRESOLIN, NEREO (Penultimo)
COMI, GIACOMO PIETRO (Corresponding)
|Parole Chiave:||neural stem cells; transplantation; neurodegenerative diseases|
|Settore Scientifico Disciplinare:||Settore MED/26 - Neurologia|
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1096/fj.05-4170fje|
|Appare nelle tipologie:||01 - Articolo su periodico|