The Brettanomyces bruxellensis species plays various roles in both the industrial and food sectors. At the biotechnological level, B. bruxellensis is considered to be a promising species for biofuel production. Its presence in alcoholic beverages can be detrimental or beneficial to the final product; B. bruxellensis can contribute to spoilage of wine and beer, but can also produce good aromas. However, little is known about its genetic characteristics and, despite the complete sequencing of several B. bruxellensis genomes and knowledge of its metabolic pathways, the toolkits for its efficient and easy genetic modification are still underdeveloped. Moreover, the different ploidy states and the high level of genotype diversity within this species makes the development of effective genetic manipulation tools challenging. This review summarizes the available tools for the genetic manipulation of B. bruxellensis and how they may be employed to improve the quality of wine and beer.

Molecular tools to exploit the biotechnological potential of brettanomyces bruxellensis : A review / A. Di Canito, R. Foschino, M. Mazzieri, I. Vigentini. - In: APPLIED SCIENCES. - ISSN 2076-3417. - 11:16(2021 Aug 09), pp. 7302.1-7302.11. [10.3390/app11167302]

Molecular tools to exploit the biotechnological potential of brettanomyces bruxellensis : A review

A. Di Canito;R. Foschino;I. Vigentini
2021-08-09

Abstract

The Brettanomyces bruxellensis species plays various roles in both the industrial and food sectors. At the biotechnological level, B. bruxellensis is considered to be a promising species for biofuel production. Its presence in alcoholic beverages can be detrimental or beneficial to the final product; B. bruxellensis can contribute to spoilage of wine and beer, but can also produce good aromas. However, little is known about its genetic characteristics and, despite the complete sequencing of several B. bruxellensis genomes and knowledge of its metabolic pathways, the toolkits for its efficient and easy genetic modification are still underdeveloped. Moreover, the different ploidy states and the high level of genotype diversity within this species makes the development of effective genetic manipulation tools challenging. This review summarizes the available tools for the genetic manipulation of B. bruxellensis and how they may be employed to improve the quality of wine and beer.
Brettanomyces bruxellensis; CRISPR/Cas9 approach; Dekkera bruxellensis; molecular biology; molecular biotechnology
Settore AGR/16 - Microbiologia Agraria
PSR2015-1720IVIGE_01 - PIANO DI SOSTEGNO ALLA RICERCA 2015-2017 - TRANSITION GRANT LINEA 1A PROGETTO "UNIMI PARTENARIATI H2020" (anno 2020) - VIGENTINI, ILEANA - PSR2015-17 - Piano di sviluppo di ricerca 2015-17 - 2020
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/864008
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