Given the pharmacological properti es and the potential role of kynurenic acid (KYNA) in human physiology and the pleiotropic activity of the neurohormone melatonin (MEL) involved in physiological and immunological functions and as regulator of antioxidant enzymes, this study aimed at evaluating the capability of Saccharomyces cerevisiae EC1118 to release tryptophan derivatives (dTRPs) from the kynurenine (KYN) and melatonin pathways. The setting up of the spectroscopic and chromatographic conditions for the quantification of the dTRPs in LC-MS/MS system, the optimization of dTRPs’ production in fermentative and whole-cell biotransformation approaches and the production of dTRPs in a soybean-based cultural medium naturally enriched in tryptophan, as a case of study, were included in the experimental plan. Variable amounts of dTRPs, with a prevalence of metabolites of the KYN pathway, were detected. The LC-MS/MS analysis showed that the compound synthesized at highest concentration is KYNA that reached 9.146 ± 0.585 mg/L in fermentation trials in a chemically defined medium at 400 mg/L TRP. Further experiments in a soybean-based medium confirm KYNA as the main dTRPs, whereas the other dTRPs reached very lower concentrations. While detectable quantities of melatonin were never observed, two MEL isomers were successfully measured in laboratory media.
Tryptophan Derivatives by Saccharomyces cerevisiae EC1118: Evaluation, Optimization, and Production in a Soybean-Based Medium / M.V. Dei Cas, I. Vigentini, S. Vitalini, A. Laganaro, M. Iriti, R.C. Paroni, R.C. Foschino. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 22:1(2021), pp. 472.1-472.20.
|Titolo:||Tryptophan Derivatives by Saccharomyces cerevisiae EC1118: Evaluation, Optimization, and Production in a Soybean-Based Medium|
DEI CAS, MICHELE VITTORIO (Co-primo)
VIGENTINI, ILEANA (Co-primo) [Conceptualization] (Corresponding)
VITALINI, SARA (Secondo)
PARONI, RITA CLARA (Penultimo)
FOSCHINO, ROBERTO CARMINE (Ultimo)
|Parole Chiave:||kynurenine pathway; MEL biosynthesis; Saccharomyces cerevisiae; yeast; tryptophan extraction; LC-MS/MS; soybean|
|Settore Scientifico Disciplinare:||Settore AGR/16 - Microbiologia Agraria|
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
Settore AGR/12 - Patologia Vegetale
|Data di pubblicazione:||2021|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/ijms22010472|
|Appare nelle tipologie:||01 - Articolo su periodico|