Substantial epidemiological evidence indicates that a diet rich in polyphenols protects against developing type 2 diabetes. The phenylethanoid glycoside verbascoside/acteoside, a widespread polyphenolic plant compound, has several biological properties including strong antioxidant, anti-inflammatory and neuroprotective activities. The aim of this research was to test the possible effects of verbascoside on pancreatic β-cells, a target never tested before. Mouse and human β-cells were incubated with verbascoside (0.8–16 µM) for up to five days and a combination of biochemical and imaging techniques were used to assess the β-cell survival and function under normal or endoplasmic reticulum (ER)-stress inducing conditions. We found a dose-dependent protective effect of verbascoside against oxidative stress in clonal and human β-cells. Mechanistic studies revealed that the polyphenol protects β-cells against ER-stress mediated dysfunctions, modulating the activation of the protein kinase RNA-like endoplasmic reticulum kinase (PERK) branch of the unfolded protein response and promoting mitochondrial dynamics. As a result, increased viability, mitochondrial function and insulin content were detected in these cells. These studies provide the evidence that verbascoside boosts the ability of β-cells to cope with ER-stress, an important contributor of β-cell dysfunction and failure in diabetic conditions and support the therapeutic potential of verbascoside in diabetes.

Verbascoside protects pancreatic β-cells against er-stress / A. Galli, P. Marciani, A. Marku, S. Ghislanzoni, F. Bertuzzi, R. Rossi, A. Di Giancamillo, M. Castagna, C. Perego. - In: BIOMEDICINES. - ISSN 2227-9059. - 8:12(2020 Dec), pp. 582.1-582.15. [10.3390/biomedicines8120582]

Verbascoside protects pancreatic β-cells against er-stress

Galli A.;Marciani P.;Marku A.;Ghislanzoni S.;Rossi R.;Di Giancamillo A.;Castagna M.;Perego C.
2020-12

Abstract

Substantial epidemiological evidence indicates that a diet rich in polyphenols protects against developing type 2 diabetes. The phenylethanoid glycoside verbascoside/acteoside, a widespread polyphenolic plant compound, has several biological properties including strong antioxidant, anti-inflammatory and neuroprotective activities. The aim of this research was to test the possible effects of verbascoside on pancreatic β-cells, a target never tested before. Mouse and human β-cells were incubated with verbascoside (0.8–16 µM) for up to five days and a combination of biochemical and imaging techniques were used to assess the β-cell survival and function under normal or endoplasmic reticulum (ER)-stress inducing conditions. We found a dose-dependent protective effect of verbascoside against oxidative stress in clonal and human β-cells. Mechanistic studies revealed that the polyphenol protects β-cells against ER-stress mediated dysfunctions, modulating the activation of the protein kinase RNA-like endoplasmic reticulum kinase (PERK) branch of the unfolded protein response and promoting mitochondrial dynamics. As a result, increased viability, mitochondrial function and insulin content were detected in these cells. These studies provide the evidence that verbascoside boosts the ability of β-cells to cope with ER-stress, an important contributor of β-cell dysfunction and failure in diabetic conditions and support the therapeutic potential of verbascoside in diabetes.
Anti-inflammatory; Diabetes; ER-stress; Insulin-producing cells; Mitochondria; Oxidative stress; PERK; Polyphenols; UPR; Verbascoside
Settore BIO/09 - Fisiologia
Settore VET/01 - Anatomia degli Animali Domestici
Settore AGR/18 - Nutrizione e Alimentazione Animale
BIOMEDICINES
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/802283
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