The supplementation of different food items with grain legumes and, in particular, with lupin has been demonstrated to provide useful health benefits, especially in the area of cardiovascular disease prevention. In this work, label free quantitative untargeted and targeted approaches based on liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) for investigating the protein profile of three pasta samples containing different percentages of narrow-leaf lupin flour were carried out. The untargeted method permitted the identification of the main acidic globulins (α-conglutin, β-conglutin, and δ-conglutin) and the comparison of their profile with raw lupin flour. The targeted method, based on High-performance liquid chromatography electrospray ionization tandem mass spectrometry HPLC-Chip-Multiple Reaction Monitoring (MRM) mode, allowed the quantification of γ-conglutin, the main hypoglycemic component of lupin protein: its concentration was around 2.25 mg/g in sample A, 2.16 mg/g in sample D, and 0.57 mg/g in sample F.
Analysis of Narrow-Leaf Lupin Proteins in Lupin-Enriched Pasta by Untargeted and Targeted Mass Spectrometry / G. Aiello, Y. Li, G. Boschin, M. Stanziale, C. Lammi, A. Arnoldi. - In: FOODS. - ISSN 2304-8158. - 9:8(2020 Aug 08), pp. 1083.1-1083.13.
|Titolo:||Analysis of Narrow-Leaf Lupin Proteins in Lupin-Enriched Pasta by Untargeted and Targeted Mass Spectrometry|
AIELLO, GILDA (Primo) (Corresponding)
LI, YUCHEN (Secondo)
LAMMI, CARMEN (Penultimo)
ARNOLDI, ANNA (Ultimo)
|Parole Chiave:||Lupinus angustifolius; food quality; functional foods; label free quantification; liquid chromatography-tandem mass spectrometry LC-MS/MS; lupin-enriched pasta; γ-conglutin|
|Settore Scientifico Disciplinare:||Settore CHIM/10 - Chimica degli Alimenti|
|Data di pubblicazione:||8-ago-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/foods9081083|
|Appare nelle tipologie:||01 - Articolo su periodico|