Raman spectroscopy has been vastly employed for the characterization of different bio-molecular species spanning from single protein to the in-vivo analysis of tissues. However, despite the huge work done, a detailed description of the Raman spectra acquired from the main classes of plasma lipoproteins is still missing. In this work, we extracted, the major classes of lipoproteins: the triacylglycerol-rich very low density lipoproteins (VLDL); the more cholesterol-rich low density lipoproteins (LDL); and the high density lipoproteins (HDL); from human plasma of six fasting healthy volunteers. The extracted lipoproteins were dried on CaF2 slides and analysed using a 633 nm laser line non-in resonance with the carotenoids present in the sample. The obtained spectra showed peaks relative to the different biomolecules composing lipoproteins: cholesterol, triglycerides, membrane lipids, carotenoids, and apolipoproteins (proteins). The intensity of the peaks from lipids and proteins are well in accordance with the measured composition of lipoproteins; but the information is acquired in a much faster way by Raman spectroscopy. Besides, Raman spectroscopy provides easily information on the levels of carotenoids and unsaturated fatty acid present in the samples. Overall, our data provide a clear comprehension of the Raman spectra from lipoproteins ad suggest that Raman spectroscopy could be a viable approach for the fast characterization of lipoproteins.

Raman spectroscopy characterization of the major classes of plasma lipoproteins / A. Ricciardi, G. Piuri, M. Della Porta, S. Mazzucchelli, A. Bonizzi, M. Truffi, M. Sevieri, R. Allevi, F. Corsi, R. Cazzola, C. Morasso. - In: VIBRATIONAL SPECTROSCOPY. - ISSN 0924-2031. - 109(2020 Jul). [10.1016/j.vibspec.2020.103073]

Raman spectroscopy characterization of the major classes of plasma lipoproteins

G. Piuri
Secondo
;
M. Della Porta;S. Mazzucchelli;A. Bonizzi;M. Truffi;M. Sevieri;R. Allevi;F. Corsi;R. Cazzola
Penultimo
;
C. Morasso
Ultimo
2020

Abstract

Raman spectroscopy has been vastly employed for the characterization of different bio-molecular species spanning from single protein to the in-vivo analysis of tissues. However, despite the huge work done, a detailed description of the Raman spectra acquired from the main classes of plasma lipoproteins is still missing. In this work, we extracted, the major classes of lipoproteins: the triacylglycerol-rich very low density lipoproteins (VLDL); the more cholesterol-rich low density lipoproteins (LDL); and the high density lipoproteins (HDL); from human plasma of six fasting healthy volunteers. The extracted lipoproteins were dried on CaF2 slides and analysed using a 633 nm laser line non-in resonance with the carotenoids present in the sample. The obtained spectra showed peaks relative to the different biomolecules composing lipoproteins: cholesterol, triglycerides, membrane lipids, carotenoids, and apolipoproteins (proteins). The intensity of the peaks from lipids and proteins are well in accordance with the measured composition of lipoproteins; but the information is acquired in a much faster way by Raman spectroscopy. Besides, Raman spectroscopy provides easily information on the levels of carotenoids and unsaturated fatty acid present in the samples. Overall, our data provide a clear comprehension of the Raman spectra from lipoproteins ad suggest that Raman spectroscopy could be a viable approach for the fast characterization of lipoproteins.
high density lipoprotein; low density lipoprotein; very low density lipoprotein; lipoprotein characterization;
Settore BIO/10 - Biochimica
Settore MED/18 - Chirurgia Generale
lug-2020
apr-2020
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/732795
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