Health benefits are routinely attributed to whey proteins, their hydrolysates and peptides based on in vitro chemical and cellular assays. The objective of this study was to track the fate of whey proteins through the upper gastrointestinal tract, their uptake across the intestinal barrier and then assess the physiological impact to downstream target cells. Simulated gastrointestinal digestion (SGID) released a selection of whey peptides some of which were transported across a Caco-2/HT-29 intestinal barrier, inhibited free radical formation in muscle and liver cells. In addition, SGID of beta-lactoglobulin resulted in the highest concentration of free amino acids (176 nM) arriving on the basolateral side of the co-culture with notable levels of branched chain and sulphur-containing amino acids. In vitro results indicate that consumption of whey proteins will deliver bioactive peptides to target cells.

Bovine whey peptides transit the intestinal barrier to reduce oxidative stress in muscle cells / A.R. Corrochano, A. Ferraretto, E. Arranz, M. Stuknytė, M. Bottani, P.M. O'Connor, P.M. Kelly, I. De Noni, V. Buckin, L. Giblin. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 288(2019), pp. 306-314.

Bovine whey peptides transit the intestinal barrier to reduce oxidative stress in muscle cells

A. Ferraretto
Secondo
;
M. Stuknytė;M. Bottani;I. De Noni;
2019

Abstract

Health benefits are routinely attributed to whey proteins, their hydrolysates and peptides based on in vitro chemical and cellular assays. The objective of this study was to track the fate of whey proteins through the upper gastrointestinal tract, their uptake across the intestinal barrier and then assess the physiological impact to downstream target cells. Simulated gastrointestinal digestion (SGID) released a selection of whey peptides some of which were transported across a Caco-2/HT-29 intestinal barrier, inhibited free radical formation in muscle and liver cells. In addition, SGID of beta-lactoglobulin resulted in the highest concentration of free amino acids (176 nM) arriving on the basolateral side of the co-culture with notable levels of branched chain and sulphur-containing amino acids. In vitro results indicate that consumption of whey proteins will deliver bioactive peptides to target cells.
Whey peptides; Gastrointestinal digestion; Bioavailability; Antioxidant activity; Muscle cells
Settore AGR/15 - Scienze e Tecnologie Alimentari
Settore MED/49 - Scienze Tecniche Dietetiche Applicate
Settore CHIM/01 - Chimica Analitica
Settore BIO/13 - Biologia Applicata
2019
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/632437
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