High-performance ion-exchange chromatography with suppressed conductivity (HPIEC-SCD) was validated for the determination of nitrite (NO2 -) and nitrate (NO3 -) in the edible part of diverse seafood species. Samples analyzed by HPIEC-SCD that were devoid of nitrite and nitrate were subjected to HRMS using a Q-exactive Orbitrap platform. As NO2 - is not detectable in Q-Exactive Orbitrap, it was necessary to perform the oxidation of NO2 - to NO3 -. Accordingly, suitability of NO2 - oxidation by H2O2 as a part of sample preparation for HPIEC-HRMS was elaborated. The difference in the concentration of NO3 - before and after H2O2 treatment was used for the evaluation of eventual NO2 - presence. The edible part of 53 fish, shrimp and bivalve species presented significant differences in NO3 - levels especially between farmed (median=44μg/g) and wild species (median=16μg/g). The highest concentration of NO3 - was found in smoked salmon samples (median=60μg/g) while NO2 - was not revealed in any of the samples studied.
Detection of nitrate and nitrite in different seafood / L. Chiesa, F. Arioli, R. Pavlovic, R. Villa, S. Panseri. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 288(2019 Aug 01), pp. 361-367. [10.1016/j.foodchem.2019.02.125]
Detection of nitrate and nitrite in different seafood
L. ChiesaPrimo
;F. ArioliSecondo
;R. Pavlovic
;R. VillaPenultimo
;S. PanseriUltimo
2019
Abstract
High-performance ion-exchange chromatography with suppressed conductivity (HPIEC-SCD) was validated for the determination of nitrite (NO2 -) and nitrate (NO3 -) in the edible part of diverse seafood species. Samples analyzed by HPIEC-SCD that were devoid of nitrite and nitrate were subjected to HRMS using a Q-exactive Orbitrap platform. As NO2 - is not detectable in Q-Exactive Orbitrap, it was necessary to perform the oxidation of NO2 - to NO3 -. Accordingly, suitability of NO2 - oxidation by H2O2 as a part of sample preparation for HPIEC-HRMS was elaborated. The difference in the concentration of NO3 - before and after H2O2 treatment was used for the evaluation of eventual NO2 - presence. The edible part of 53 fish, shrimp and bivalve species presented significant differences in NO3 - levels especially between farmed (median=44μg/g) and wild species (median=16μg/g). The highest concentration of NO3 - was found in smoked salmon samples (median=60μg/g) while NO2 - was not revealed in any of the samples studied.File | Dimensione | Formato | |
---|---|---|---|
109 Detection of nitrate and nitrite in different seafood.pdf
Open Access dal 23/11/2020
Tipologia:
Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione
768.19 kB
Formato
Adobe PDF
|
768.19 kB | Adobe PDF | Visualizza/Apri |
1-s2.0-S0308814619304911-main.pdf
accesso riservato
Tipologia:
Publisher's version/PDF
Dimensione
556.05 kB
Formato
Adobe PDF
|
556.05 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.