This study investigated the capacity of an anthocyanin-rich fraction (ACN-RF) from blueberry, single anthocyanins (cyanidin, delphinidin and malvidin-3-glucoside; Cy, Dp and Mv-3-glc) and related metabolites (protocatechuic, gallic and syringic acid; PrA, GA and SA) to resolve an inflammation-driven adhesion of monocytes (THP-1) on endothelial cell (HUVECs) and secretion of cell adhesion molecules E-selectin and vascular cell adhesion molecule 1 (VCAM-1). The adhesion of THP-1 to HUVECs was induced by tumour necrosis factor α (TNF-α, 100 ng mL-1). Subsequently, ACN-RF, single ACNs and metabolites (from 0.01 to 10 μg mL-1) were incubated for 24 h. The adhesion was measured in a fluorescence spectrophotometer. E-selectin and VCAM-1 were quantified by ELISA. No toxicological effects were observed for the compounds and the doses tested. ACN-RF and Mv-3-glc reducedTHP-1 adhesion at all the concentrations with the maximum effect at 10 μg/ml (-60.2% for ACNs and-33.9% for Mv-3-glc). Cy-3-glc decreased the adhesion by about 41.8% at 10 μg mL-1, while PrA and GA reduced the adhesion of THP-1 to HUVECs both at 1 and at 10 μg mL-1 (-29.5% and -44.3% for PrA, respectively, and -18.0%and -59.3% for GA, respectively). At the same concentrations a significant reduction of E-selectin, but notVCAM-1 levels, was documented. No effect was observed following Dp-3-glc and SA supplementation. Overall, ACNs and metabolites seem to resolve, in a dose-dependent manner, the inflammation-driven adhesion of THP-1 to HUVECs by decreasing E-selectin concentrations. Interestingly, Mv-3-glc was active at physiologically relevant concentrations.
Anthocyanins and metabolites resolve TNF-α-mediated production of E-selectin and adhesion of monocytes to endothelial cells / C. Del Bo', M. Marino, P. Riso, P. Møller, M. Porrini. - In: CHEMICO-BIOLOGICAL INTERACTIONS. - ISSN 0009-2797. - 300(2019 Feb 25), pp. 49-55.
|Titolo:||Anthocyanins and metabolites resolve TNF-α-mediated production of E-selectin and adhesion of monocytes to endothelial cells|
DEL BO', CRISTIAN (Primo)
MARINO, MIRKO (Secondo)
RISO, PATRIZIA (Corresponding)
PORRINI, MARISA (Ultimo)
|Parole Chiave:||Anthocyanins; Atherogenesis; Cell culture; E-selectin; Metabolites; VCAM-1|
|Settore Scientifico Disciplinare:||Settore MED/49 - Scienze Tecniche Dietetiche Applicate|
|Progetto:||PIANO DI SOSTEGNO ALLA RICERCA 2015-2017 - LINEA 2 "DOTAZIONE ANNUALE PER ATTIVITA' ISTITUZIONALE" (ANNO 2016)|
|Data di pubblicazione:||25-feb-2019|
|Data ahead of print / Data di stampa:||3-gen-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.cbi.2019.01.002|
|Appare nelle tipologie:||01 - Articolo su periodico|