High performance liquid chromatography, coupled with a benchtop Q-Exactive Orbitrap high-resolution mass spectrometer, was successfully applied for the determination of 24 target antibiotics (selected beta-lactams, tetracyclines, fluoroquinolones, sulfonamids, phenicols, macrolides, cephalosporins, lincosamides, diaminopyrimidine) in fish matrices. The Q-Exactive parameters were carefully studied to accomplish the best compromise between a suitable scan speed and selectivity, considering the restrictions associated with generic sample preparation methodology. Retention time, an exact mass with tolerance of 2 ppm and data-dependent MS2 spectra were the main identifiers. The method was validated through specificity, linearity, recovery, intraand inter-day repeatability, decision limit (CCα) and detection capability (CCβ), according to 2002/657/EC. The values of CCα and CCβ ranged from 29.2 to 36.8 and 32.5 to 48.9, respectively, while overall recovery ranged from 91.1 to 105.6%. Fifty fish samples were analysed, showing the sporadic incidence of enrofloxacin, chlortetracycline, oxytetracycline, amoxicillin and trimethoprim, albeit below the maximum residual levels.
Validated multiclass targeted determination of antibiotics in fish with high performance liquid chromatography–benchtop quadrupole orbitrap hybrid mass spectrometry / L. Chiesa, S. Panseri, E. Pasquale, R. Malandra, R. Pavlovic, F. Arioli. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 258(2018), pp. 222-230.
|Titolo:||Validated multiclass targeted determination of antibiotics in fish with high performance liquid chromatography–benchtop quadrupole orbitrap hybrid mass spectrometry|
CHIESA, LUCA MARIA (Primo)
PANSERI, SARA (Secondo)
PAVLOVIC, RADMILA (Corresponding)
ARIOLI, FRANCESCO (Ultimo)
|Parole Chiave:||q-Exactive Orbitrap; Fish; Antibiotics; Food safety|
|Settore Scientifico Disciplinare:||Settore VET/04 - Ispezione degli Alimenti di Origine Animale|
Settore VET/07 - Farmacologia e Tossicologia Veterinaria
|Data di pubblicazione:||2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.foodchem.2018.03.072|
|Appare nelle tipologie:||01 - Articolo su periodico|
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