Copper sulphate forms in piglet diets : microbiota, intestinal morphology and enteric nervous system glial cells

The objective of this study was to evaluate dietary supplementation with different copper sulphate (CuSO4) forms on small intestine microanatomy and large intestine microbiota. Ninety weaned piglets were divided into three experimental groups: control diet (CTR), with no added CuSO4and diets supplemented with 150 ppm of CuSO4in protected (150P) and unprotected form (150UP). After 18 days of dietary treatment, six piglets per treatment were randomly selected and sacrificed. Duodenum villi length and crypt depths were higher (P < 0.001) in the animals fed 150UP than other groups. Glial fibrillary acidic protein (GFAP), a marker for enteric glial cells, was unaffected by dietary treatments. The total bacteria and Enterobacteriaceae bacteria counts were lower (P < 0.05) in cecum of animals fed 150P in comparison with the other two groups. In the colon the Streptococci spp were lower (P < 0.001) in both CuSO4supplemented groups than controls. The obtained results revealed a modulation of intestinal structure and microbiota exerted by the studied CuSO4dietary supplementation. The present data show that dietary supplementation with 150UP in the first period post-weaning may assist in restoring the gut morphology, improving duodenal structure.