A hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl). The resulting hydrolysate was fractionated by semipreparative RP-HPLC, and the purified fractions were tested as inhibitors of angiotensin converting enzyme (ACE). Mono- and bidimensional NMR experiments and LC-MS analyses led to the identification of four potentially bioactive peptides, i.e. GVLY, IEE, LGV, and RVR. They were prepared by solid-phase synthesis, and tested for ACE-inhibitory activity. The IC50 values were GVLY 16 ± 1.5 μM, LGV 145 ± 13 μM, and RVR 526 ± 33 μM, confirming that hemp seed may be a valuable source of hypotensive peptides.
New ACE-Inhibitory Peptides from Hemp Seed (Cannabis sativa L.) Proteins / O. Lara, G. Boschin, T. Recca, C. Morelli, R. Laura, P. Francescato, A. Arnoldi, G. Speranza. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - 65:48(2017 Dec 06), pp. 10482-10488.
|Titolo:||New ACE-Inhibitory Peptides from Hemp Seed (Cannabis sativa L.) Proteins|
BOSCHIN, GIOVANNA (Secondo) (Corresponding)
ARNOLDI, ANNA (Penultimo)
SPERANZA, GIOVANNA (Ultimo) (Corresponding)
|Parole Chiave:||ACE-inhibitors; Cannabis sativa L.; LC-MS/MS; bioactive peptides; hemp seed protein hydrolysate|
|Settore Scientifico Disciplinare:||Settore CHIM/06 - Chimica Organica|
Settore CHIM/10 - Chimica degli Alimenti
|Data di pubblicazione:||6-dic-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1021/acs.jafc.7b04522|
|Appare nelle tipologie:||01 - Articolo su periodico|