The seed of industrial hemp is an underexploited protein source. In view of a possible use in functional foods, a hempseed protein concentrate was hydrolyzed with pepsin, trypsin, pancreatin, or a mixture of these enzymes. A detailed peptidomic analysis using data-dependent acquisition showed that the numbers of peptides identified ranged from 90 belonging to 33 parent proteins in the peptic hydrolysate to 9 belonging to 6 proteins in the pancreatin digest. The peptic and tryptic hydrolysates resulted to be the most efficient inhibitors of 3-hydroxymethyl-coenzyme A reductase activity when tested on the catalytic domain of the enzyme. Using the open access tools PeptideRanker and BIOPEP, a list of potentially bioactive peptides was generated: the alleged activities included the antioxidant property, the glucose uptake stimulating activity, the inhibition of dipeptidyl peptidase-IV and angiotensin-converting enzyme I.
Exploration of Potentially Bioactive Peptides Generated from the Enzymatic Hydrolysis of Hempseed Proteins / G. Aiello, C. Lammi, G. Boschin, C. Zanoni, A. Arnoldi. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - 65:47(2017 Nov 29), pp. 10174-10184.
Titolo: | Exploration of Potentially Bioactive Peptides Generated from the Enzymatic Hydrolysis of Hempseed Proteins |
Autori: | AIELLO, GILDA (Primo) LAMMI, CARMEN (Secondo) ZANONI, CHIARA (Penultimo) ARNOLDI, ANNA (Ultimo) (Corresponding) |
Parole Chiave: | Cannabis sativa; HMGCoAR; LC−MS/MS; bioactive peptides; hempseed hydrolysates; peptidomics |
Settore Scientifico Disciplinare: | Settore CHIM/10 - Chimica degli Alimenti |
Data di pubblicazione: | 29-nov-2017 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1021/acs.jafc.7b03590 |
Appare nelle tipologie: | 01 - Articolo su periodico |
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2017_Hemp_3_ revised 28sept.pdf | Pre-print (manoscritto inviato all'editore) | Open Access Visualizza/Apri | ||
2017 Aiello Canapa idrolizzati.pdf | Publisher's version/PDF | Administrator Richiedi una copia |