A water soluble derivative (2) of topopyrones was selected for NMR studies directed to elucidate the mode of binding with specific oligonucleotides. Topopyrone 2 can intercalate into the CG base pairs, but the residence time into the double helix is very short and a fast chemical exchange averaging occurs at room temperature between the free and bound species. The equilibria involved become slow below room temperature, thus allowing to measure a mean lifetime of the complex of ca. 7 ms at 15 degrees C. Structural models of the complex with d(CGTACG)(2) were developed on the basis of DOSY, 2D NOESY and (31)P NMR experiments. Topopyrone 2 presents a strong tendency to self-associate. In the presence of oligonucleotide a certain number of ligand molecules are found to externally stack to the double-helix, in addition to a small fraction of the same ligand intercalated. The external binding to the ionic surface of the phosphoribose chains may thus represents the first step of the intercalation process.
Interaction between double-helix DNA fragments and a new topopyrone acting as a topoisomerase I poison / L. Scaglioni, S. Mazzini, R. Mondelli, S. Dallavalle, S. Gattinoni, S. Tinelli, G.L. Beretta, F. Zunino, E.M. Ragg. - In: BIOORGANIC & MEDICINAL CHEMISTRY. - ISSN 0968-0896. - 17:2(2009), pp. 484-491.
|Titolo:||Interaction between double-helix DNA fragments and a new topopyrone acting as a topoisomerase I poison|
SCAGLIONI, LEONARDO (Primo)
MAZZINI, STEFANIA (Secondo)
RAGG, ENZIO MARIA (Ultimo)
|Settore Scientifico Disciplinare:||Settore CHIM/06 - Chimica Organica|
|Data di pubblicazione:||2009|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.bmc.2008.12.005|
|Appare nelle tipologie:||01 - Articolo su periodico|