Background The significance of the presence of anti-gliadin antibodies in patients affected by celiac disease is still unclear. It is hypothesized that gliadin deamidation, catalysed by transglutaminase, plays a role in favoring the antigen presentation. Aim To determine the immunoreactivity of anti-gliadin antibodies from untreated celiac patients to transglutaminase deamidated gliadins. Materials and methods Gliadins from wheat flour underwent enzymatic digestion and were deamidated or cysteamine-transamidated by transglutaminase. Immunoreactivity of anti-gliadin antibodies from untreated adult celiac patients sera was evaluated by means of a competitive enzyme-linked immunosorbent assay (ELISA) method. Results Gliadin deamidation increased antibodies immunoreactivity from 25% to 50% while cysteamine incorporation into the gliadin peptides resulted in an immunoreactivity decrease. Conclusions Increased immunoreactivity of transglutaminase deamidated gliadins tested with anti-gliadin antibodies from untreated adult celiac patients supports the hypothesis of a pivotal role of gliadin deamidation in the pathomechanism of celiac disease.
Immunoreactivity of antibodies against transglutaminase-deamidated gliadins in adult celiac disease / M.L. Falini, L. Elli, R. Caramanico, M.T. Bardella, C. Terrani, L.R. Roncoroni, L. Doneda, F. Forlani. - In: DIGESTIVE DISEASES AND SCIENCES. - ISSN 0163-2116. - 53:10(2008 Oct), pp. 2697-2701.
|Titolo:||Immunoreactivity of antibodies against transglutaminase-deamidated gliadins in adult celiac disease|
FALINI, MARIA LETIZIA (Primo)
ELLI, LUCA (Secondo)
DONEDA, LUIGIA (Penultimo)
FORLANI, FABIO (Ultimo)
|Parole Chiave:||Anti-gliadin antibodies; Celiac disease; Gliadin; Transglutaminase|
|Settore Scientifico Disciplinare:||Settore MED/12 - Gastroenterologia|
Settore BIO/13 - Biologia Applicata
|Data di pubblicazione:||ott-2008|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s10620-007-0191-9|
|Appare nelle tipologie:||01 - Articolo su periodico|