Rubinstein-Taybi syndrome (RSTS) is a rare congenital neurodevelopmental disorder characterized by growth deficiency, skeletal abnormalities, dysmorphic features, and intellectual disability. Causative mutations in CREBBP and EP300 genes have been identified in similar to 55% and similar to 8% of affected individuals. To date, only 28 EP300 alterations in 29 RSTS clinically described patients have been reported. EP300 analysis of 22 CREBBP-negative RSTS patients from our cohort led us to identify six novel mutations: a 376-kb deletion depleting EP300 gene; an exons 17-19 deletion (c.(3141+1_3142-1)_(3590+1_3591-1)del/p.(Ile1047 Serfs*30)); two stop mutations, (c.3829A>T/p.(Lys1277*) and c.4585C>T/p.(Arg1529*)); a splicing mutation (c.1878-12A>G/p.(Ala627Glnfs*11)), and a duplication (c.4640dupA/p.(Asn1547Lysfs*3)). All EP300-mutated individuals show a mild RSTS phenotype and peculiar findings including maternal gestosis, skin manifestation, especially nevi or keloids, back malformations, and a behavior predisposing to anxiety. Furthermore, the patient carrying the complete EP300 deletion does not show a markedly severe clinical picture, even if a more composite phenotype was noticed. By characterizing six novel EP300-mutated patients, this study provides further insights into the EP300-specific clinical presentation and expands the mutational repertoire including the first case of a whole gene deletion. These new data will enhance EP300-mutated cases identification highlighting distinctive features and will improve the clinical practice allowing a better genotype-phenotype correlation.
From Whole Gene Deletion to Point Mutations of EP300-Positive Rubinstein-Taybi Patients : New Insights into the Mutational Spectrum and Peculiar Clinical Hallmarks / G. Negri, P. Magini, D. Milani, P. Colapietro, D. Rusconi, E. Scarano, M.T. Bonati, M. Priolo, M. Crippa, L. Mazzanti, A. Wischmeijer, F. Tamburrino, T. Pippucci, P. Finelli, L. Larizza, C. Gervasini. - In: HUMAN MUTATION. - ISSN 1059-7794. - 37:2(2016 Feb), pp. 175-183.
|Titolo:||From Whole Gene Deletion to Point Mutations of EP300-Positive Rubinstein-Taybi Patients : New Insights into the Mutational Spectrum and Peculiar Clinical Hallmarks|
NEGRI, GLORIA (Primo)
LARIZZA, LIDIA (Penultimo)
|Parole Chiave:||deletion; EP300; genotype–phenotype correlation; Rubinstein–Taybi syndrome; RSTS|
|Settore Scientifico Disciplinare:||Settore MED/03 - Genetica Medica|
|Data di pubblicazione:||feb-2016|
|Data ahead of print / Data di stampa:||4-nov-2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/humu.22922|
|Appare nelle tipologie:||01 - Articolo su periodico|