A rapid, specific and simple procedure is proposed for the determination of free malonaldehyde (MA) contained in fish tissue. The method is the optimization of the reaction of MA with 2,3-diaminonaphthalene to afford a naphtodiazepinium ion that present a UV absorption at 311 nm, useful for MA determination by HPLC with UV detection. The reaction proceeds in the presence of 25% acetonitrile at 37 degrees C in 20 min at pH 2 using 2,4-pentanedione as internal standard. The method has been applied to homogenized samples of canned mackerel fillets that were treated with 2,3-diaminonaphthalene in an acidic aqueous:acetonitrile mixture. The produced naphtodiazepinium ion was extracted in acetonitrile by a salting-out homogeneous liquid-liquid extraction. A standard calibration was carried out in the range 0.625-10 nmol/g. The reliability of the procedure is demonstrated by linearity (r(2) = 0.998), limit of detection (0.16 nmol/g), limit of quantification (0.22 nmol/g), repeatibility (RSD 5.57%), and intermediate precision (RSD 8.92%).
Improved determination of malonaldehyde by high-performance liquid chromatography with UV detection as iaminonaphthalene derivative / S. Panseri, L. M. Chiesa, A. Brizzolari, E. Passerò, P. A. Biondi. - In: JOURNAL OF CHROMATOGRAPHY. B. - ISSN 1570-0232. - 976(2015 Jan 22), pp. 91-95. [10.1016/j.jchromb.2014.11.017]
Improved determination of malonaldehyde by high-performance liquid chromatography with UV detection as iaminonaphthalene derivative
S. PanseriPrimo
;L. M. ChiesaSecondo
;A. Brizzolari;E. PasseròPenultimo
;P. A. BiondiUltimo
2015
Abstract
A rapid, specific and simple procedure is proposed for the determination of free malonaldehyde (MA) contained in fish tissue. The method is the optimization of the reaction of MA with 2,3-diaminonaphthalene to afford a naphtodiazepinium ion that present a UV absorption at 311 nm, useful for MA determination by HPLC with UV detection. The reaction proceeds in the presence of 25% acetonitrile at 37 degrees C in 20 min at pH 2 using 2,4-pentanedione as internal standard. The method has been applied to homogenized samples of canned mackerel fillets that were treated with 2,3-diaminonaphthalene in an acidic aqueous:acetonitrile mixture. The produced naphtodiazepinium ion was extracted in acetonitrile by a salting-out homogeneous liquid-liquid extraction. A standard calibration was carried out in the range 0.625-10 nmol/g. The reliability of the procedure is demonstrated by linearity (r(2) = 0.998), limit of detection (0.16 nmol/g), limit of quantification (0.22 nmol/g), repeatibility (RSD 5.57%), and intermediate precision (RSD 8.92%).File | Dimensione | Formato | |
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