BACKGROUND: NF-Y is a transcription factor that recognizes with high specificity and affinity the widespread CCAAT box promoter element. It is formed by three subunits: NF-YA and the NF-YB/NF-YC- heterodimer containing histone fold domains (HFDs). We previously identified a large NF-Y gene family in Arabidopsis thaliana, composed of 29 members, and characterized their expression patterns in various plant tissues. METHODS: We used yeast Two-hybrids assays (Y2H), pull-down and Electrophoretic Mobility Shift Assay (EMSA) in vitro experiments with recombinant proteins to dissect AtNF-YB/AtNF-YC interactions and DNA-binding with different AtNF-YAs. RESULTS: Consistent with robust conservation within HFDs, we show that heterodimerization is possible among all histone-like subunits, including the divergent and related LEC1/AtNF-YB9 and L1L/AtNF-YB6 required for embryo development. DNA-binding to a consensus CCAAT box was investigated with specific AtNF-YB/AtNF-YC combinations and observed with some, but not all AtNF-YA subunits. CONCLUSIONS: Our results highlight (i) the conserved heterodimerization capacity of AtNF-Y histone-like subunits, and (ii) the different affinities of AtNF-YAs for the CCAAT sequence. Because of the general expansion of NF-Y genes in plants, these results most likely apply to other species.
Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits / V. Calvenzani, B. Testoni, G. Gusmaroli, M. Lorenzo, N. Gnesutta, K. Petroni, R. Mantovani, C. Tonelli. - In: PLOS ONE. - ISSN 1932-6203. - 7:8(2012 Aug), p. e42902.1.
|Titolo:||Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits|
CALVENZANI, VALENTINA (Primo)
MANTOVANI, ROBERTO (Penultimo)
TONELLI, CHIARA (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/18 - Genetica|
|Data di pubblicazione:||ago-2012|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1371/journal.pone.0042902|
|Appare nelle tipologie:||01 - Articolo su periodico|