Specific regions in three genes coding for aminopeptidases C and N, and a trypsin-like serine protease were selected as species-specific primer sequences for rapid and reliable identification of Lactobacillus helveticus strains. The PCR procedures carried out gave specific 524-, 726- and 918-bp amplificates, with DNA isolated from L. helveticus. No PCR product was generated for closely related bacteria. The amplification products were also screened for their species specificity in dot blot hybridization with representatives of the most closely related genera and species and a number of other bacterial species.
Specific identification of Lactobacillus helveticus by PCR with pepC, pepN and htrA targeted primers / M. Fortina, G. Ricci, D. Mora, C. Parini, P. Manachini. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - 198:1(2001), pp. 85-89.
Specific identification of Lactobacillus helveticus by PCR with pepC, pepN and htrA targeted primers
M. FortinaPrimo
;G. RicciSecondo
;D. Mora;C. PariniPenultimo
;P. ManachiniUltimo
2001
Abstract
Specific regions in three genes coding for aminopeptidases C and N, and a trypsin-like serine protease were selected as species-specific primer sequences for rapid and reliable identification of Lactobacillus helveticus strains. The PCR procedures carried out gave specific 524-, 726- and 918-bp amplificates, with DNA isolated from L. helveticus. No PCR product was generated for closely related bacteria. The amplification products were also screened for their species specificity in dot blot hybridization with representatives of the most closely related genera and species and a number of other bacterial species.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
