A high degree of genetic polymorphism among P. acidilactici strains was highlighted by a multilocus typing approach analysing several housekeeping genes and by sampling the whole genome using random amplified polymorphic DNA (RAPD) fingerprint analysis performed by using a single primer pedA gene targeted in low-stringency amplification conditions. Restriction fragment length polymorphism of the rpoC, ldhD/L and mle genes, and a modified RAPD analysis, permitted the grouping of Pediococcus acidilactici strains in seven genotypes (I–VII). Genotypic results obtained by analysing housekeeping genes involved in the transcription/translation machinery and in primary metabolism were supported by phylogenetic analysis based on the partial 16S rDNA sequencing of a reference strain of each of the seven clusters obtained. Three of the seven genotypes detected showed relationships with pediocin AcH/PA-1 production and carbohydrate fermentation patterns: all pediocin-producing and sucrose-positive strains were grouped in genotype VII, melibiose-, sucrose- and raffinose-positive strains in genotype VI, and arabinose-positive strains in genotype V.
Genomic subpopulations within the species Pediococcus acidilactici detected by multilocus typing analysis: relationships between pediocin AcH/PA-1 producing and non-producing strains / D. Mora, M. Fortina, C. Parini, D. Daffonchio, P. Manachini. - In: MICROBIOLOGY. - ISSN 1350-0872. - 146:8(2000), pp. 2027-2038.
Titolo: | Genomic subpopulations within the species Pediococcus acidilactici detected by multilocus typing analysis: relationships between pediocin AcH/PA-1 producing and non-producing strains |
Autori: | MORA, DIEGO (Primo) FORTINA, MARIA GRAZIA (Secondo) DAFFONCHIO, DANIELE GIUSEPPE (Penultimo) MANACHINI, PIER LUIGI (Ultimo) |
Parole Chiave: | Intraspecific genetic variability; Multilocus typing; Pediocin AcH/PA-1; Pediococcus acidilactici |
Settore Scientifico Disciplinare: | Settore AGR/16 - Microbiologia Agraria |
Data di pubblicazione: | 2000 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1099/00221287-146-8-2027 |
Appare nelle tipologie: | 01 - Articolo su periodico |