A method for histamine determination in fish and fish products by capillary electrophoresis and ion-pair liquid chromatography has been developed. Histamine was extracted from different fish samples with 10% trichloroacetic acid, and the filtrate was directly applied to capillary electrophoresis apparatus. A preliminary purification through a solid phases extraction cartridge (Sep-pak C18) was necessary for HPLC analysis. Histamine was detected ‘on-line’ at 210 nm by both approaches. HPLC procedure allowed accurate (±2.2%) and repeatable (±1.6%) determination of histamine in fish products at appropriate levels (20–100 mg kg−1) to meet current regulations
Determination of histamine in fish products by capillary electrophoresis and ion-pair liquid chromatography with diode-array detection / C. Gardana, P. Pietta, S. Ciappellano, G. Testolin. - In: JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE. - ISSN 0022-5142. - 79:1(1999), pp. 91-94.
Determination of histamine in fish products by capillary electrophoresis and ion-pair liquid chromatography with diode-array detection
C. GardanaPrimo
;S. CiappellanoPenultimo
;G. TestolinUltimo
1999
Abstract
A method for histamine determination in fish and fish products by capillary electrophoresis and ion-pair liquid chromatography has been developed. Histamine was extracted from different fish samples with 10% trichloroacetic acid, and the filtrate was directly applied to capillary electrophoresis apparatus. A preliminary purification through a solid phases extraction cartridge (Sep-pak C18) was necessary for HPLC analysis. Histamine was detected ‘on-line’ at 210 nm by both approaches. HPLC procedure allowed accurate (±2.2%) and repeatable (±1.6%) determination of histamine in fish products at appropriate levels (20–100 mg kg−1) to meet current regulations
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