A new reverse-phase HPLC method that uses 'nonaggressive' eluents and a coupled revelation system based on diode-array and dual electrode electrochemical detection is described. This method is suitable for direct measurement of polyphenols in wines and offers distinct advantages in terms of selectivity and sensitivity when compared with alternative HPLC methods, based on UV-vis or PDA detection, since multiple features of the analytescan be checked during sample analysis. Good peak resolution was obtained following direct injection of a 20 μL sample, and more than 20 different analytes were identified by spectral comparison with known standards and by their electrochemical behavior at potentials of +0.4 and +0.8V (vs Ag/AgCl).
A new HPLC method for the determination of polyphenols in wine that uses non aggressive eluent and a coupled revelation system / O. Brenna, S. Buratti, M.S. Cosio, S. Mannino. - In: ELECTROANALYSIS. - ISSN 1040-0397. - 10:17(1998), pp. 1204-1207. [10.1002/(SICI)1521-4109(199811)10:17<1204::AID-ELAN1204>3.0.CO;2-I]
A new HPLC method for the determination of polyphenols in wine that uses non aggressive eluent and a coupled revelation system
O. BrennaPrimo
;S. BurattiSecondo
;M.S. CosioPenultimo
;S. ManninoUltimo
1998
Abstract
A new reverse-phase HPLC method that uses 'nonaggressive' eluents and a coupled revelation system based on diode-array and dual electrode electrochemical detection is described. This method is suitable for direct measurement of polyphenols in wines and offers distinct advantages in terms of selectivity and sensitivity when compared with alternative HPLC methods, based on UV-vis or PDA detection, since multiple features of the analytescan be checked during sample analysis. Good peak resolution was obtained following direct injection of a 20 μL sample, and more than 20 different analytes were identified by spectral comparison with known standards and by their electrochemical behavior at potentials of +0.4 and +0.8V (vs Ag/AgCl).
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