Nanofibrous membranes have been produced by electrospinning to develop first generation glucose biosensors. The direct immobilization of glucose oxidase onto the polyamide nanofibrous surfaces by drop coating revealed a simple and efficient method for the development of sensitive, stable, and reproducible electrochemical biosensors. The biosensor showed a linear response over the range 1–9×10−3 glucose (R2=0.9997) with a sensitivity of 1.11 μA/mM and a limit of detection of 2.5×10−6 M (S/N=3). The uncertainty of repeatability was 2% (RSD%, n=30). After one month of storage, the signal decreased of 35%. The recovery of glucose, evaluated in real samples of honey, was 98% (RSD%=1%, n=3).

Nylon nanofibrous biosensors for glucose determination / M.M. Scampicchio, A. Arecchi, A. Bianco, A. Bulbarello, C. Bertarelli, S. Mannino. - In: ELECTROANALYSIS. - ISSN 1040-0397. - 22:10(2010 May), pp. 1056-1060. [10.1002/elan.200900446]

Nylon nanofibrous biosensors for glucose determination

M.M. Scampicchio;A. Arecchi;A. Bianco;A. Bulbarello;C. Bertarelli;S. Mannino
2010-05

Abstract

Nanofibrous membranes have been produced by electrospinning to develop first generation glucose biosensors. The direct immobilization of glucose oxidase onto the polyamide nanofibrous surfaces by drop coating revealed a simple and efficient method for the development of sensitive, stable, and reproducible electrochemical biosensors. The biosensor showed a linear response over the range 1–9×10−3 glucose (R2=0.9997) with a sensitivity of 1.11 μA/mM and a limit of detection of 2.5×10−6 M (S/N=3). The uncertainty of repeatability was 2% (RSD%, n=30). After one month of storage, the signal decreased of 35%. The recovery of glucose, evaluated in real samples of honey, was 98% (RSD%=1%, n=3).
Amperometric detection; Biosensors; Electrospinning; Glucose oxidase; Membranes; Nanofibrous membrane; Nylon-6
Settore AGR/15 - Scienze e Tecnologie Alimentari
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/171405
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